Detailed information of TA system TA098005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4825634..4826259
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6N65_RS25805
• Protein ID: WP_032206177.1
• Coordinates: 4825634..4826032 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6N65_RS25810
• Protein ID: WP_000450524.1
• Coordinates: 4826032..4826259 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS25785	4821513..4821713	+	201	WP_000383836.1	YpfN family protein	-
C6N65_RS25790	4821823..4822520	-	698	Protein_4690	esterase	-
C6N65_RS25795	4822594..4824609	-	2016	WP_032206176.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6N65_RS25800	4824624..4825487	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
C6N65_RS25805	4825634..4826032	-	399	WP_032206177.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6N65_RS25810	4826032..4826259	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6N65_RS25815	4826414..4827127	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6N65_RS25820	4827340..4828374	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6N65_RS25825	4828391..4829269	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6N65_RS25830	4829415..4829987	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6N65_RS25835	4829987..4830457	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14852.17 Da
• Isoelectric Point: 9.2216

>T98005 WP_032206177.1 NZ_CP027325:c4826032-4825634 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPGGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98005 NZ_CP027325:c4826032-4825634 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGGTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98005 WP_000450524.1 NZ_CP027325:c4826259-4826032 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98005 NZ_CP027325:c4826259-4826032 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33