Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3600075..3600713 Replicon chromosome
Accession NZ_CP027325
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6N65_RS19305 Protein ID WP_000813794.1
Coordinates 3600537..3600713 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6N65_RS19300 Protein ID WP_001270286.1
Coordinates 3600075..3600491 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS19280 3595227..3596168 - 942 WP_032206936.1 ABC transporter permease -
C6N65_RS19285 3596169..3597182 - 1014 WP_032206935.1 ABC transporter ATP-binding protein -
C6N65_RS19290 3597200..3598345 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
C6N65_RS19295 3598590..3599996 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C6N65_RS19300 3600075..3600491 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6N65_RS19305 3600537..3600713 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6N65_RS19310 3600935..3601165 + 231 WP_071887485.1 YncJ family protein -
C6N65_RS19315 3601257..3603218 - 1962 Protein_3495 23S rRNA 5-hydroxycytidine C2501 synthase -
C6N65_RS19320 3603291..3603827 - 537 WP_032206933.1 DNA-binding transcriptional regulator SutR -
C6N65_RS19325 3603880..3605094 + 1215 WP_071887553.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3605134..3606282 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97995 WP_000813794.1 NZ_CP027325:c3600713-3600537 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97995 NZ_CP027325:c3600713-3600537 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGACGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT97995 WP_001270286.1 NZ_CP027325:c3600491-3600075 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97995 NZ_CP027325:c3600491-3600075 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References