Detailed information of TA system TA097993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2966904..2967385
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: C6N65_RS15485
• Protein ID: WP_021568727.1
• Coordinates: 2966904..2967194 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: C6N65_RS15490
• Protein ID: WP_000108762.1
• Coordinates: 2967194..2967385 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS15450	2961965..2962207	-	243	WP_000273151.1	DUF4224 domain-containing protein	-
C6N65_RS15455	2962275..2964749	-	2475	WP_032206202.1	exonuclease	-
C6N65_RS15460	2964843..2965034	-	192	WP_001098307.1	DUF1482 family protein	-
C6N65_RS15465	2965031..2965219	-	189	WP_000413705.1	cell division inhibition protein DicB	-
C6N65_RS15475	2965932..2966378	+	447	WP_000373334.1	hypothetical protein	-
C6N65_RS15480	2966477..2966629	-	153	WP_000367377.1	DUF1391 family protein	-
C6N65_RS15485	2966904..2967194	-	291	WP_021568727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N65_RS15490	2967194..2967385	-	192	WP_000108762.1	hypothetical protein	Antitoxin
C6N65_RS15495	2967403..2967903	-	501	WP_021568728.1	helix-turn-helix transcriptional regulator	-
C6N65_RS15500	2968011..2968274	+	264	WP_001048459.1	helix-turn-helix domain-containing protein	-
C6N65_RS15505	2968271..2968696	+	426	WP_000693918.1	Rha family transcriptional regulator	-
C6N65_RS15510	2968768..2969838	+	1071	WP_001262367.1	hypothetical protein	-
C6N65_RS15515	2969879..2970304	+	426	WP_047087727.1	DUF977 family protein	-
C6N65_RS15520	2970358..2970717	+	360	WP_032206196.1	hypothetical protein	-
C6N65_RS15525	2970763..2970975	+	213	WP_032206194.1	hypothetical protein	-
C6N65_RS15530	2971011..2971844	+	834	WP_032206192.1	hypothetical protein	-
C6N65_RS15535	2971954..2972058	+	105	WP_001278459.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	espFu/tccP	2960762..3006306	45544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11105.96 Da
• Isoelectric Point: 9.9854

>T97993 WP_021568727.1 NZ_CP027325:c2967194-2966904 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLSEHPYLYPPSERALGLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T97993 NZ_CP027325:c2967194-2966904 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCGCGTGATGATTTGCGTCAGATCGTAGCCTATATTGCCAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTTCTGAACATCCTTACCTGTATCCACCAAGCG
AAAGAGCTCTAGGCCTTCGTGAGATAGTGACCCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGGCAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7075.91 Da
• Isoelectric Point: 4.0270

>AT97993 WP_000108762.1 NZ_CP027325:c2967385-2967194 [Escherichia coli]
MSTVLSPIISEFETIEQENSYNEWLRTKVAASLADPRPAIPHDEVMAEMENLIAQIASTNRSE

Nucleotide

• Length: 192 bp

>AT97993 NZ_CP027325:c2967385-2967194 [Escherichia coli]
ATGAGTACAGTCCTTTCCCCGATAATTTCAGAATTTGAAACAATCGAACAAGAAAACAGCTATAACGAATGGTTACGCAC
CAAAGTGGCAGCAAGCCTCGCAGACCCCCGTCCTGCAATTCCGCATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CGCAAATAGCTTCGACGAACAGGAGTGAGTAA