Detailed information of TA system TA097989

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1724428..1725131
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8T6BCS1
• Locus tag: C6N65_RS08930
• Protein ID: WP_001616568.1
• Coordinates: 1724790..1725131 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C6N65_RS08925
• Protein ID: WP_032208435.1
• Coordinates: 1724428..1724769 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS08885	1719887..1720600	+	714	WP_032208428.1	transcriptional regulator	-
C6N65_RS08890	1720597..1721232	+	636	WP_001616559.1	hypothetical protein	-
C6N65_RS08895	1721271..1721726	+	456	WP_032208430.1	hypothetical protein	-
C6N65_RS08900	1721723..1722172	+	450	WP_032208432.1	hypothetical protein	-
C6N65_RS08905	1722242..1722472	+	231	WP_024188497.1	DUF905 domain-containing protein	-
C6N65_RS08910	1722570..1723391	+	822	WP_032208434.1	DUF945 domain-containing protein	-
C6N65_RS08915	1723422..1723862	+	441	WP_001616563.1	antirestriction protein	-
C6N65_RS08920	1723875..1724417	+	543	WP_001616564.1	DNA repair protein RadC	-
C6N65_RS08925	1724428..1724769	+	342	WP_032208435.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6N65_RS08930	1724790..1725131	+	342	WP_001616568.1	TA system toxin CbtA family protein	Toxin
C6N65_RS08935	1725379..1726386	+	1008	WP_001616572.1	restriction endonuclease	-
C6N65_RS08945	1727026..1728212	+	1187	Protein_1614	IS3-like element IS2 family transposase	-
C6N65_RS08955	1728604..1728726	+	123	WP_001300013.1	hypothetical protein	-
C6N65_RS08960	1728734..1729714	-	981	WP_032208436.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	1697397..1734752	37355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12746.75 Da
• Isoelectric Point: 9.6548

>T97989 WP_001616568.1 NZ_CP027325:1724790-1725131 [Escherichia coli]
MKTLPATTPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T97989 NZ_CP027325:1724790-1725131 [Escherichia coli]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTAAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.46 Da
• Isoelectric Point: 6.4660

>AT97989 WP_032208435.1 NZ_CP027325:1724428-1724769 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLRLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYYNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT97989 NZ_CP027325:1724428-1724769 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAGGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACTACAATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA