Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1355550..1356181 Replicon chromosome
Accession NZ_CP027325
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6N65_RS06945 Protein ID WP_001259385.1
Coordinates 1355550..1355825 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6N65_RS06950 Protein ID WP_000593555.1
Coordinates 1355822..1356181 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS06930 1350554..1351621 + 1068 WP_032207298.1 HlyD family secretion protein -
C6N65_RS06935 1351618..1354353 + 2736 WP_032207297.1 ribosome-associated ATPase/putative transporter RbbA -
C6N65_RS06940 1354353..1355477 + 1125 WP_032207295.1 ABC transporter permease -
C6N65_RS06945 1355550..1355825 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6N65_RS06950 1355822..1356181 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6N65_RS06955 1356301..1356702 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6N65_RS06960 1356708..1357514 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6N65_RS06965 1357511..1358275 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C6N65_RS06970 1358275..1359108 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6N65_RS06975 1359105..1360049 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1345280..1356702 11422


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T97988 WP_001259385.1 NZ_CP027325:1355550-1355825 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97988 NZ_CP027325:1355550-1355825 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT97988 WP_000593555.1 NZ_CP027325:1355822-1356181 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97988 NZ_CP027325:1355822-1356181 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTATGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References