Detailed information of TA system TA097986

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 572927..573373
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: C6N65_RS03005
• Protein ID: WP_072033237.1
• Coordinates: 572927..573049 (-)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: C6N65_RS03010
• Protein ID: WP_071887523.1
• Coordinates: 573077..573373 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS02985	570127..570696	-	570	WP_047619141.1	inovirus Gp2 family protein	-
C6N65_RS02990	571060..572222	+	1163	WP_085947598.1	IS3-like element IS3 family transposase	-
C6N65_RS02995	572256..572896	-	641	Protein_541	transposase	-
C6N65_RS03005	572927..573049	-	123	WP_072033237.1	GhoT/OrtT family toxin	Toxin
C6N65_RS03010	573077..573373	-	297	WP_071887523.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
C6N65_RS03015	573600..573872	-	273	WP_032206017.1	N-acetyltransferase	-
C6N65_RS03020	573884..574101	-	218	Protein_545	(4Fe-4S)-binding protein	-
C6N65_RS03025	574282..575913	+	1632	WP_001216477.1	sensor histidine kinase	-
C6N65_RS03030	575910..576629	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-36)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4687.74 Da
• Isoelectric Point: 10.8827

>T97986 WP_072033237.1 NZ_CP027325:c573049-572927 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSRLAP

Nucleotide

• Length: 123 bp

>T97986 NZ_CP027325:c573049-572927 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTAGACTGGCCCCCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11455.92 Da
• Isoelectric Point: 4.1705

>AT97986 WP_071887523.1 NZ_CP027325:c573373-573077 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGTPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT97986 NZ_CP027325:c573373-573077 [Escherichia coli]
ATGGAAGGTAAAAACAAATTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGACACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG