Detailed information of TA system TA097982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 112742..113276
• Replicon: plasmid unnamed
• Accession: NZ_CP027324
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6N24_RS29440
• Protein ID: WP_106894699.1
• Coordinates: 112986..113276 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A136X611
• Locus tag: C6N24_RS29435
• Protein ID: WP_000108831.1
• Coordinates: 112742..112996 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS29395	108434..109075	-	642	WP_089611238.1	transcription termination factor NusG	-
C6N24_RS29405	109777..110649	+	873	WP_199852859.1	incFII family plasmid replication initiator RepA	-
C6N24_RS29425	111644..111907	+	264	WP_106894697.1	plasmid stabilization protein	-
C6N24_RS29430	111897..112196	+	300	WP_106894698.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N24_RS29435	112742..112996	+	255	WP_000108831.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C6N24_RS29440	112986..113276	+	291	WP_106894699.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N24_RS29445	113380..113973	+	594	WP_106894700.1	serine acetyltransferase	-
C6N24_RS29450	114073..114690	+	618	WP_106894701.1	hypothetical protein	-
C6N24_RS29455	114715..115008	+	294	WP_106894702.1	hypothetical protein	-
C6N24_RS29460	115192..116348	+	1157	WP_089611701.1	IS3 family transposase	-
C6N24_RS29470	116684..117550	+	867	WP_106894703.1	radical SAM protein	-
C6N24_RS29475	117547..118275	+	729	WP_106894704.1	deoxynucleoside kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..127667	127667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-58)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11401.38 Da
• Isoelectric Point: 10.6932

>T97982 WP_106894699.1 NZ_CP027324:112986-113276 [Escherichia coli]
MTFNIDFDERAFKEWKKLDKTIRDQFKKKLRKLQHNPCTGATRLHGDLAGCFKIKLRASGFRLIYKVIDEEIVIWVIAVG
KRERSNVYNIASERMR

Nucleotide

• Length: 291 bp

>T97982 NZ_CP027324:112986-113276 [Escherichia coli]
ATGACCTTTAATATCGACTTCGATGAAAGAGCCTTTAAAGAGTGGAAGAAACTGGACAAGACAATACGTGACCAGTTTAA
AAAGAAACTCAGGAAACTACAGCATAATCCCTGTACTGGTGCTACCAGGCTTCATGGTGACCTGGCTGGATGTTTTAAAA
TAAAACTAAGAGCATCTGGGTTTCGGCTTATTTATAAGGTCATTGATGAGGAAATTGTTATATGGGTGATTGCTGTAGGT
AAACGAGAGCGGAGTAACGTTTATAATATCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9870.22 Da
• Isoelectric Point: 4.5498

>AT97982 WP_000108831.1 NZ_CP027324:112742-112996 [Escherichia coli]
MSTVQIRVDEELKKNAWQVFEKLNLSPSEALRLFLRYVAENEKLPFSEVSVVVSDNDEDEDILNIVRERLKNPARRIRVN
IDDL

Nucleotide

• Length: 255 bp

>AT97982 NZ_CP027324:112742-112996 [Escherichia coli]
ATGAGTACTGTTCAAATTCGTGTAGATGAAGAACTGAAGAAAAATGCCTGGCAGGTCTTCGAAAAGCTCAATCTTTCACC
TTCAGAGGCCCTCCGCCTTTTTCTCCGTTATGTGGCTGAAAATGAAAAACTTCCTTTCAGTGAAGTGTCTGTTGTGGTCA
GTGACAATGATGAGGATGAAGATATTCTGAATATTGTCAGAGAACGGCTGAAAAATCCTGCCAGACGCATCAGGGTGAAT
ATTGATGACCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A136X611