Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 61172..61746 Replicon plasmid unnamed
Accession NZ_CP027324
Organism Escherichia coli strain 2013C-3033

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag C6N24_RS29110 Protein ID WP_089610697.1
Coordinates 61172..61546 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag C6N24_RS29115 Protein ID WP_089610695.1
Coordinates 61543..61746 (-) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N24_RS29075 56716..57201 - 486 WP_106894660.1 thermonuclease family protein -
C6N24_RS29080 57362..57985 + 624 WP_106894661.1 DNA-binding protein -
C6N24_RS29085 58073..58528 + 456 WP_106894662.1 DUF2569 family protein -
C6N24_RS29090 58728..60263 + 1536 WP_199852861.1 phosphoethanolamine transferase -
C6N24_RS29095 60356..60598 - 243 WP_106894664.1 hypothetical protein -
C6N24_RS29100 60931..61083 + 153 WP_059331012.1 Hok/Gef family protein -
C6N24_RS29110 61172..61546 - 375 WP_089610697.1 type II toxin-antitoxin system VapC family toxin Toxin
C6N24_RS29115 61543..61746 - 204 WP_089610695.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
C6N24_RS29120 62190..62342 + 153 WP_001367784.1 Hok/Gef family protein -
C6N24_RS29130 62429..62917 - 489 WP_106894665.1 GNAT family N-acetyltransferase -
C6N24_RS29135 62923..63204 - 282 WP_089610691.1 DUF1778 domain-containing protein -
C6N24_RS29140 63361..64011 - 651 WP_106894666.1 plasmid IncI1-type surface exclusion protein ExcA -
C6N24_RS29145 64099..66264 - 2166 WP_106894667.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..127667 127667


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-44)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13732.12 Da        Isoelectric Point: 6.4688

>T97981 WP_089610697.1 NZ_CP027324:c61546-61172 [Escherichia coli]
MILVDTSVWVDHFKNKNDTLIQLLLSDCVLMHPMILAEIACGTPPAPRQQTLSNLNLLPKSHEATMTEVLAFIENEKLFG
SGCGLVDITLLASTRITPGARLWTLDKRLSRLADILNIGFQPVH

Download         Length: 375 bp

>T97981 NZ_CP027324:c61546-61172 [Escherichia coli]
ATGATATTGGTTGATACGTCTGTATGGGTTGATCATTTTAAAAATAAAAATGATACATTGATTCAACTATTGCTGTCGGA
TTGTGTTCTTATGCATCCAATGATTCTGGCTGAAATCGCATGTGGCACACCGCCAGCACCCAGACAACAGACATTAAGTA
ATCTGAACTTGTTGCCGAAAAGCCATGAGGCAACAATGACCGAAGTTTTGGCATTTATCGAAAATGAAAAATTATTTGGT
TCAGGTTGTGGGCTGGTCGATATAACACTACTGGCGTCAACGCGAATTACTCCCGGAGCCAGACTATGGACATTAGATAA
ACGCCTGTCACGGCTGGCAGATATTCTTAATATTGGATTTCAGCCAGTACATTAA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7461.48 Da        Isoelectric Point: 4.7085

>AT97981 WP_089610695.1 NZ_CP027324:c61746-61543 [Escherichia coli]
MRTTVTIDDALYAQALDMAEPGMDKSDIFREAVKTFIRVQAAKRLASLGGTAPDMEITSRRREDTVE

Download         Length: 204 bp

>AT97981 NZ_CP027324:c61746-61543 [Escherichia coli]
ATGAGAACAACTGTAACAATCGACGATGCTCTGTACGCACAGGCTCTGGACATGGCTGAACCCGGTATGGATAAATCAGA
TATTTTCCGTGAAGCAGTGAAAACATTTATCCGTGTACAGGCAGCAAAACGTCTGGCATCACTCGGTGGAACTGCTCCAG
ATATGGAAATAACATCTCGCAGACGTGAGGACACAGTGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References