Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3773598..3774229 Replicon chromosome
Accession NZ_CP027323
Organism Escherichia coli strain 2013C-3033

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag C6N24_RS20160 Protein ID WP_001259388.1
Coordinates 3773954..3774229 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6N24_RS20155 Protein ID WP_000593555.1
Coordinates 3773598..3773957 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N24_RS20130 3769730..3770674 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
C6N24_RS20135 3770671..3771504 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6N24_RS20140 3771504..3772268 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
C6N24_RS20145 3772265..3773071 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
C6N24_RS20150 3773077..3773478 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6N24_RS20155 3773598..3773957 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6N24_RS20160 3773954..3774229 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
C6N24_RS20165 3774302..3775426 - 1125 WP_001314210.1 ABC transporter permease -
C6N24_RS20170 3775426..3778161 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
C6N24_RS20175 3778158..3779225 - 1068 WP_000361485.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T97975 WP_001259388.1 NZ_CP027323:c3774229-3773954 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97975 NZ_CP027323:c3774229-3773954 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT97975 WP_000593555.1 NZ_CP027323:c3773957-3773598 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97975 NZ_CP027323:c3773957-3773598 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References