Detailed information of TA system TA097970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2638486..2639111
• Replicon: chromosome
• Accession: NZ_CP027323
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6N24_RS14170
• Protein ID: WP_000911330.1
• Coordinates: 2638486..2638884 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6N24_RS14175
• Protein ID: WP_000450524.1
• Coordinates: 2638884..2639111 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS14155	2634791..2635372	-	582	Protein_2602	esterase	-
C6N24_RS14160	2635446..2637461	-	2016	WP_106894503.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6N24_RS14165	2637476..2638339	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6N24_RS14170	2638486..2638884	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6N24_RS14175	2638884..2639111	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6N24_RS14180	2639264..2639977	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6N24_RS14185	2640190..2641224	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6N24_RS14190	2641241..2642119	-	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6N24_RS14195	2642265..2642837	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6N24_RS14200	2642837..2643307	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2629956..2639111	9155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97970 WP_000911330.1 NZ_CP027323:c2638884-2638486 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97970 NZ_CP027323:c2638884-2638486 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97970 WP_000450524.1 NZ_CP027323:c2639111-2638884 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97970 NZ_CP027323:c2639111-2638884 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33