Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47414..48057 Replicon plasmid unnamed2
Accession NZ_CP027321
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag AAG01_RS23775 Protein ID WP_001044768.1
Coordinates 47414..47830 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag AAG01_RS23780 Protein ID WP_001261287.1
Coordinates 47827..48057 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS23745 42653..43000 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
AAG01_RS23750 42997..43671 - 675 WP_001341423.1 IS66-like element accessory protein TnpA -
AAG01_RS23755 43755..44111 - 357 Protein_40 tyrosine-type recombinase/integrase -
AAG01_RS23760 44239..45099 + 861 WP_000704534.1 alpha/beta hydrolase -
AAG01_RS23770 46707..47252 + 546 WP_001165114.1 hypothetical protein -
AAG01_RS23775 47414..47830 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
AAG01_RS23780 47827..48057 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AAG01_RS23785 48617..49030 + 414 WP_000465041.1 hypothetical protein -
AAG01_RS23790 49032..49814 + 783 WP_001164205.1 site-specific integrase -
AAG01_RS23795 49987..50340 + 354 WP_000864810.1 colicin M immunity protein -
AAG01_RS23800 50348..50539 - 192 Protein_49 lipid II-degrading bacteriocin -
AAG01_RS23805 50543..50686 - 144 Protein_50 transcriptional regulator -
AAG01_RS23810 50753..52192 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / toxB / hlyD / hlyB / hlyA / hlyC / espP 1..84276 84276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T97955 WP_001044768.1 NZ_CP027321:c47830-47414 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T97955 NZ_CP027321:c47830-47414 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT97955 WP_001261287.1 NZ_CP027321:c48057-47827 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT97955 NZ_CP027321:c48057-47827 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References