Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 43026..43627 Replicon plasmid unnamed1
Accession NZ_CP027320
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag AAG01_RS23375 Protein ID WP_001216045.1
Coordinates 43247..43627 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag AAG01_RS23370 Protein ID WP_001190712.1
Coordinates 43026..43247 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS23335 38425..38718 + 294 WP_000269004.1 hypothetical protein -
AAG01_RS23340 38725..39099 + 375 WP_001749397.1 hypothetical protein -
AAG01_RS23345 39081..40013 + 933 WP_001749396.1 hypothetical protein -
AAG01_RS23350 40010..40372 + 363 WP_001261543.1 hypothetical protein -
AAG01_RS23355 41448..41804 - 357 WP_001062545.1 hypothetical protein -
AAG01_RS23360 41805..42389 - 585 WP_001749394.1 hypothetical protein -
AAG01_RS23365 42564..42953 + 390 WP_000506730.1 S24 family peptidase -
AAG01_RS23370 43026..43247 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AAG01_RS23375 43247..43627 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
AAG01_RS23380 43632..43811 + 180 WP_000113018.1 hypothetical protein -
AAG01_RS23385 43839..44882 + 1044 WP_000648833.1 DUF968 domain-containing protein -
AAG01_RS23390 44971..45423 + 453 WP_001312282.1 late promoter-activating protein (Gp10) -
AAG01_RS23395 45510..46703 + 1194 WP_062858117.1 hypothetical protein -
AAG01_RS23400 46703..48187 + 1485 WP_000124159.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..78854 78854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T97954 WP_001216045.1 NZ_CP027320:43247-43627 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T97954 NZ_CP027320:43247-43627 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCAGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT97954 WP_001190712.1 NZ_CP027320:43026-43247 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT97954 NZ_CP027320:43026-43247 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References