Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4075374..4075992 Replicon chromosome
Accession NZ_CP027319
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AAG01_RS20020 Protein ID WP_001291435.1
Coordinates 4075374..4075592 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AAG01_RS20025 Protein ID WP_000344800.1
Coordinates 4075618..4075992 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS19985 (4070663) 4070663..4071235 + 573 WP_000779831.1 YbaY family lipoprotein -
AAG01_RS19990 (4071266) 4071266..4071577 - 312 WP_000409911.1 MGMT family protein -
AAG01_RS20000 (4071956) 4071956..4072309 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AAG01_RS20005 (4072351) 4072351..4073901 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AAG01_RS20010 (4074065) 4074065..4074535 - 471 WP_000136192.1 YlaC family protein -
AAG01_RS20015 (4074651) 4074651..4075202 - 552 WP_000102564.1 maltose O-acetyltransferase -
AAG01_RS20020 (4075374) 4075374..4075592 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AAG01_RS20025 (4075618) 4075618..4075992 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AAG01_RS20030 (4076538) 4076538..4079687 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AAG01_RS20035 (4079710) 4079710..4080903 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97951 WP_001291435.1 NZ_CP027319:c4075592-4075374 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T97951 NZ_CP027319:c4075592-4075374 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT97951 WP_000344800.1 NZ_CP027319:c4075992-4075618 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT97951 NZ_CP027319:c4075992-4075618 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References