Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3645317..3645867 Replicon chromosome
Accession NZ_CP027319
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag AAG01_RS18025 Protein ID WP_001160974.1
Coordinates 3645553..3645867 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag AAG01_RS18020 Protein ID WP_000125566.1
Coordinates 3645317..3645550 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS17995 (3640388) 3640388..3640675 + 288 WP_000203748.1 ferredoxin-like protein FixX -
AAG01_RS18000 (3640733) 3640733..3642064 + 1332 WP_001183192.1 MFS transporter -
AAG01_RS18005 (3642175) 3642175..3642705 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
AAG01_RS18010 (3642698) 3642698..3644560 + 1863 WP_000377137.1 glutathione-regulated potassium-efflux system protein KefC -
AAG01_RS18015 (3644752) 3644752..3645231 + 480 WP_024221573.1 type 3 dihydrofolate reductase -
AAG01_RS18020 (3645317) 3645317..3645550 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AAG01_RS18025 (3645553) 3645553..3645867 + 315 WP_001160974.1 CcdB family protein Toxin
AAG01_RS18030 (3645864) 3645864..3646712 - 849 WP_000257203.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
AAG01_RS18035 (3646719) 3646719..3647096 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
AAG01_RS18040 (3647099) 3647099..3647920 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
AAG01_RS18045 (3647917) 3647917..3648906 - 990 WP_000241240.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
AAG01_RS18050 (3648906) 3648906..3650192 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T97950 WP_001160974.1 NZ_CP027319:3645553-3645867 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T97950 NZ_CP027319:3645553-3645867 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT97950 WP_000125566.1 NZ_CP027319:3645317-3645550 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT97950 NZ_CP027319:3645317-3645550 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References