Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3281818..3282315 Replicon chromosome
Accession NZ_CP027319
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AAG01_RS16395 Protein ID WP_001173343.1
Coordinates 3282142..3282315 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AAG01_RS16390 Protein ID WP_000398619.1
Coordinates 3281818..3282114 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS16370 (3278549) 3278549..3279268 - 720 WP_000611288.1 two-component system response regulator DcuR -
AAG01_RS16375 (3279265) 3279265..3280896 - 1632 WP_022581427.1 sensor histidine kinase -
AAG01_RS16380 (3281077) 3281077..3281307 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
AAG01_RS16385 (3281319) 3281319..3281591 + 273 WP_000405653.1 GNAT family N-acetyltransferase -
AAG01_RS16390 (3281818) 3281818..3282114 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AAG01_RS16395 (3282142) 3282142..3282315 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AAG01_RS16400 (3282434) 3282434..3283951 - 1518 WP_001295074.1 lysine--tRNA ligase -
AAG01_RS16405 (3284188) 3284188..3285645 - 1458 WP_000856822.1 dipeptide/tripeptide permease DtpC -
AAG01_RS16410 (3285790) 3285790..3287003 + 1214 WP_136952279.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T97946 WP_001173343.1 NZ_CP027319:3282142-3282315 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T97946 NZ_CP027319:3282142-3282315 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT97946 WP_000398619.1 NZ_CP027319:3281818-3282114 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT97946 NZ_CP027319:3281818-3282114 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References