Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2461322..2461953 Replicon chromosome
Accession NZ_CP027319
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag AAG01_RS12545 Protein ID WP_001259387.1
Coordinates 2461678..2461953 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AAG01_RS12540 Protein ID WP_000593555.1
Coordinates 2461322..2461681 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS12510 (2456434) 2456434..2456907 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
AAG01_RS12515 (2456904) 2456904..2457185 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
AAG01_RS12520 (2457262) 2457262..2458620 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
AAG01_RS12525 (2458613) 2458613..2460121 + 1509 WP_001400448.1 FGGY-family carbohydrate kinase -
AAG01_RS12530 (2460111) 2460111..2460380 + 270 WP_000933061.1 HPr family phosphocarrier protein -
AAG01_RS12535 (2460412) 2460412..2461272 + 861 WP_001131788.1 class II aldolase -
AAG01_RS12540 (2461322) 2461322..2461681 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AAG01_RS12545 (2461678) 2461678..2461953 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
AAG01_RS12550 (2462026) 2462026..2463150 - 1125 WP_001314210.1 ABC transporter permease -
AAG01_RS12555 (2463150) 2463150..2465885 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
AAG01_RS12560 (2465882) 2465882..2466949 - 1068 WP_000361517.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T97939 WP_001259387.1 NZ_CP027319:c2461953-2461678 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97939 NZ_CP027319:c2461953-2461678 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT97939 WP_000593555.1 NZ_CP027319:c2461681-2461322 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97939 NZ_CP027319:c2461681-2461322 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References