Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 642117..642755 Replicon chromosome
Accession NZ_CP027319
Organism Escherichia coli strain 2014C-3084

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AAG01_RS03490 Protein ID WP_000813794.1
Coordinates 642117..642293 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AAG01_RS03495 Protein ID WP_001270286.1
Coordinates 642339..642755 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAG01_RS03470 (637739) 637739..638911 - 1173 WP_001236321.1 BenE family transporter YdcO -
AAG01_RS03475 (639003) 639003..639539 + 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
AAG01_RS03480 (639612) 639612..641573 + 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
AAG01_RS03485 (641665) 641665..641895 - 231 WP_000494244.1 YncJ family protein -
AAG01_RS03490 (642117) 642117..642293 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AAG01_RS03495 (642339) 642339..642755 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AAG01_RS03500 (642834) 642834..644240 + 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
AAG01_RS03505 (644486) 644486..645631 + 1146 WP_000335342.1 ABC transporter substrate-binding protein -
AAG01_RS03510 (645649) 645649..646662 + 1014 WP_000220414.1 ABC transporter ATP-binding protein -
AAG01_RS03515 (646663) 646663..647604 + 942 WP_001251328.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97929 WP_000813794.1 NZ_CP027319:642117-642293 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97929 NZ_CP027319:642117-642293 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT97929 WP_001270286.1 NZ_CP027319:642339-642755 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97929 NZ_CP027319:642339-642755 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References