Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 5206539..5207010 Replicon chromosome
Accession NZ_CP027317
Organism Escherichia coli strain 2015C-3107

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6N21_RS27395 Protein ID WP_001303511.1
Coordinates 5206539..5206817 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6N21_RS27400 Protein ID WP_001302048.1
Coordinates 5206819..5207010 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N21_RS27365 5202027..5202296 - 270 WP_000003742.1 excisionase -
C6N21_RS27370 5202358..5204829 - 2472 WP_000048560.1 exonuclease -
C6N21_RS27375 5204953..5205174 - 222 WP_000560212.1 cell division protein FtsZ -
C6N21_RS27385 5205887..5206186 - 300 WP_001240334.1 hypothetical protein -
C6N21_RS27395 5206539..5206817 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6N21_RS27400 5206819..5207010 - 192 WP_001302048.1 hypothetical protein Antitoxin
C6N21_RS27405 5207031..5207402 - 372 WP_001169687.1 hypothetical protein -
C6N21_RS27410 5207500..5207802 + 303 WP_000172738.1 transcriptional regulator -
C6N21_RS27415 5207799..5208224 + 426 WP_000693944.1 Rha family transcriptional regulator -
C6N21_RS27420 5208247..5209209 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
C6N21_RS27425 5209250..5209672 + 423 WP_001151187.1 DUF977 family protein -
C6N21_RS27430 5209778..5209990 + 213 WP_000935423.1 hypothetical protein -
C6N21_RS27435 5210023..5210241 + 219 WP_000209148.1 DUF4014 family protein -
C6N21_RS29465 5210243..5210401 + 159 WP_001370098.1 hypothetical protein -
C6N21_RS27440 5210405..5211961 - 1557 WP_001066422.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 5197092..5245268 48176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T97917 WP_001303511.1 NZ_CP027317:c5206817-5206539 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T97917 NZ_CP027317:c5206817-5206539 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT97917 WP_001302048.1 NZ_CP027317:c5207010-5206819 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT97917 NZ_CP027317:c5207010-5206819 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References