Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3581528..3582025 Replicon chromosome
Accession NZ_CP027317
Organism Escherichia coli strain 2015C-3107

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6N21_RS19005 Protein ID WP_001173343.1
Coordinates 3581852..3582025 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W5Y3H9
Locus tag C6N21_RS19000 Protein ID WP_071532590.1
Coordinates 3581528..3581824 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N21_RS18975 3578259..3578978 - 720 WP_000611288.1 two-component system response regulator DcuR -
C6N21_RS18980 3578975..3580606 - 1632 WP_001216468.1 sensor histidine kinase -
C6N21_RS18985 3580787..3581017 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
C6N21_RS18990 3581029..3581301 + 273 WP_000405647.1 N-acetyltransferase -
C6N21_RS19000 3581528..3581824 + 297 WP_071532590.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6N21_RS19005 3581852..3582025 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6N21_RS19010 3582144..3583661 - 1518 WP_001295082.1 lysine--tRNA ligase -
C6N21_RS19015 3583898..3585355 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T97912 WP_001173343.1 NZ_CP027317:3581852-3582025 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T97912 NZ_CP027317:3581852-3582025 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.04 Da        Isoelectric Point: 4.2931

>AT97912 WP_071532590.1 NZ_CP027317:3581528-3581824 [Escherichia coli]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT97912 NZ_CP027317:3581528-3581824 [Escherichia coli]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y3H9

References