Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2786612..2787243 Replicon chromosome
Accession NZ_CP027317
Organism Escherichia coli strain 2015C-3107

Toxin (Protein)


Gene name hicA Uniprot ID A0A377MSE5
Locus tag C6N21_RS14945 Protein ID WP_001259383.1
Coordinates 2786968..2787243 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6N21_RS14940 Protein ID WP_000593555.1
Coordinates 2786612..2786971 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N21_RS14915 2782744..2783688 + 945 WP_000947089.1 nickel ABC transporter permease subunit NikB -
C6N21_RS14920 2783685..2784518 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
C6N21_RS14925 2784518..2785282 + 765 WP_001136244.1 nickel import ATP-binding protein NikD -
C6N21_RS14930 2785279..2786085 + 807 WP_000173673.1 nickel import ATP-binding protein NikE -
C6N21_RS14935 2786091..2786492 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6N21_RS14940 2786612..2786971 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6N21_RS14945 2786968..2787243 - 276 WP_001259383.1 type II toxin-antitoxin system HicA family toxin Toxin
C6N21_RS14950 2787316..2788440 - 1125 WP_001343434.1 ABC transporter permease -
C6N21_RS14955 2788440..2791175 - 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
C6N21_RS14960 2791172..2792239 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10209.98 Da        Isoelectric Point: 10.7076

>T97909 WP_001259383.1 NZ_CP027317:c2787243-2786968 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGPRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97909 NZ_CP027317:c2787243-2786968 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTCCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT97909 WP_000593555.1 NZ_CP027317:c2786971-2786612 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97909 NZ_CP027317:c2786971-2786612 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MSE5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References