Detailed information of TA system TA097906

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2271828..2272612
• Replicon: chromosome
• Accession: NZ_CP027317
• Organism: Escherichia coli strain 2015C-3107

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: C6N21_RS12145
• Protein ID: WP_000854717.1
• Coordinates: 2272286..2272612 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6N21_RS12140
• Protein ID: WP_001285487.1
• Coordinates: 2271828..2272196 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N21_RS12100	2267704..2267877	-	174	Protein_2249	N-acetyltransferase	-
C6N21_RS12105	2268368..2268574	+	207	WP_000632607.1	AlpA family transcriptional regulator	-
C6N21_RS12110	2268673..2269850	+	1178	WP_085968792.1	IS3 family transposase	-
C6N21_RS12115	2269856..2270164	+	309	Protein_2252	DUF932 domain-containing protein	-
C6N21_RS12120	2270164..2270409	+	246	WP_032212706.1	hypothetical protein	-
C6N21_RS12125	2270503..2270976	+	474	WP_000855068.1	antirestriction protein	-
C6N21_RS12130	2270992..2271468	+	477	WP_001186728.1	RadC family protein	-
C6N21_RS12135	2271531..2271752	+	222	WP_000692312.1	DUF987 domain-containing protein	-
C6N21_RS12140	2271828..2272196	+	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6N21_RS12145	2272286..2272612	+	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
C6N21_RS12150	2272660..2273148	+	489	WP_000761730.1	hypothetical protein	-
C6N21_RS12155	2273160..2273357	+	198	WP_000839243.1	DUF957 domain-containing protein	-
C6N21_RS12160	2273442..2274284	+	843	WP_024174205.1	DUF4942 domain-containing protein	-
C6N21_RS12165	2274627..2275163	-	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
C6N21_RS12170	2275165..2276343	-	1179	WP_000094965.1	type II secretion system protein GspL	-
C6N21_RS12175	2276340..2277317	-	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T97906 WP_000854717.1 NZ_CP027317:2272286-2272612 [Escherichia coli]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T97906 NZ_CP027317:2272286-2272612 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT97906 WP_001285487.1 NZ_CP027317:2271828-2272196 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT97906 NZ_CP027317:2271828-2272196 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8