Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2131152..2131812 Replicon chromosome
Accession NZ_CP027317
Organism Escherichia coli strain 2015C-3107

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag C6N21_RS11305 Protein ID WP_000244778.1
Coordinates 2131152..2131565 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6N21_RS11310 Protein ID WP_000354046.1
Coordinates 2131546..2131812 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N21_RS11285 2127116..2128849 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
C6N21_RS11290 2128855..2129565 - 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6N21_RS11295 2129589..2130485 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6N21_RS11300 2130597..2131118 + 522 WP_001055874.1 flavodoxin FldB -
C6N21_RS11305 2131152..2131565 - 414 WP_000244778.1 protein YgfX Toxin
C6N21_RS11310 2131546..2131812 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6N21_RS11315 2132055..2133035 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6N21_RS11320 2133112..2133771 - 660 WP_000250274.1 hemolysin III family protein -
C6N21_RS11325 2133935..2134246 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
C6N21_RS11330 2134291..2135724 + 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
C6N21_RS11335 2135781..2136524 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T97904 WP_000244778.1 NZ_CP027317:c2131565-2131152 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T97904 NZ_CP027317:c2131565-2131152 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97904 WP_000354046.1 NZ_CP027317:c2131812-2131546 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97904 NZ_CP027317:c2131812-2131546 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References