Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4613690..4614344 Replicon chromosome
Accession NZ_CP027312
Organism Escherichia coli strain 2013C-3181

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag MJ73_RS23925 Protein ID WP_000244772.1
Coordinates 4613937..4614344 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MJ73_RS23920 Protein ID WP_000354046.1
Coordinates 4613690..4613956 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ73_RS23895 4608859..4609602 + 744 WP_000951964.1 SDR family oxidoreductase -
MJ73_RS23900 4609659..4611092 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
MJ73_RS23905 4611137..4611448 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MJ73_RS23910 4611612..4612271 + 660 WP_000250274.1 hemolysin III family protein -
MJ73_RS23915 4612467..4613447 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MJ73_RS23920 4613690..4613956 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MJ73_RS23925 4613937..4614344 + 408 WP_000244772.1 protein YgfX Toxin
MJ73_RS23930 4614384..4614905 - 522 WP_001055874.1 flavodoxin FldB -
MJ73_RS23935 4615017..4615913 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MJ73_RS23940 4615938..4616648 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MJ73_RS23945 4616654..4618387 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T97883 WP_000244772.1 NZ_CP027312:4613937-4614344 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T97883 NZ_CP027312:4613937-4614344 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97883 WP_000354046.1 NZ_CP027312:4613690-4613956 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97883 NZ_CP027312:4613690-4613956 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References