Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3947551..3948182 Replicon chromosome
Accession NZ_CP027312
Organism Escherichia coli strain 2013C-3181

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag MJ73_RS20290 Protein ID WP_001259388.1
Coordinates 3947551..3947826 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MJ73_RS20295 Protein ID WP_000593555.1
Coordinates 3947823..3948182 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ73_RS20275 3942555..3943622 + 1068 WP_000361485.1 HlyD family secretion protein -
MJ73_RS20280 3943619..3946354 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
MJ73_RS20285 3946354..3947478 + 1125 WP_001314210.1 ABC transporter permease -
MJ73_RS20290 3947551..3947826 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
MJ73_RS20295 3947823..3948182 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MJ73_RS20300 3948232..3949092 - 861 WP_021554581.1 class II aldolase -
MJ73_RS20305 3949124..3949393 - 270 WP_021554580.1 HPr family phosphocarrier protein -
MJ73_RS20310 3949383..3950891 - 1509 WP_021554579.1 xylulose kinase -
MJ73_RS20315 3950884..3952242 - 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
MJ73_RS20320 3952319..3952600 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
MJ73_RS20325 3952597..3953070 - 474 WP_021554578.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3939952..3948182 8230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T97880 WP_001259388.1 NZ_CP027312:3947551-3947826 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T97880 NZ_CP027312:3947551-3947826 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT97880 WP_000593555.1 NZ_CP027312:3947823-3948182 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT97880 NZ_CP027312:3947823-3948182 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCTGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References