Detailed information of TA system TA097876

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3135035..3135833
• Replicon: chromosome
• Accession: NZ_CP027312
• Organism: Escherichia coli strain 2013C-3181

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0P7PH46
• Locus tag: MJ73_RS16290
• Protein ID: WP_000854896.1
• Coordinates: 3135456..3135833 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A234XUW5
• Locus tag: MJ73_RS16285
• Protein ID: WP_024175300.1
• Coordinates: 3135035..3135409 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ73_RS16260	3132379..3132612	+	234	WP_001117568.1	DUF905 family protein	-
MJ73_RS16265	3132703..3133521	+	819	WP_033884686.1	DUF945 domain-containing protein	-
MJ73_RS16270	3133611..3134096	+	486	WP_000214386.1	antirestriction protein	-
MJ73_RS16275	3134112..3134588	+	477	WP_001186727.1	RadC family protein	-
MJ73_RS16280	3134651..3134872	+	222	WP_000692358.1	DUF987 domain-containing protein	-
MJ73_RS16285	3135035..3135409	+	375	WP_024175300.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ73_RS16290	3135456..3135833	+	378	WP_000854896.1	TA system toxin CbtA family protein	Toxin
MJ73_RS16295	3135830..3136318	+	489	WP_000761662.1	hypothetical protein	-
MJ73_RS16300	3136330..3136527	+	198	WP_000839256.1	DUF957 domain-containing protein	-
MJ73_RS16305	3136612..3137478	+	867	WP_000014510.1	DUF4942 domain-containing protein	-
MJ73_RS16310	3137550..3137813	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
MJ73_RS16315	3137810..3138136	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MJ73_RS16325	3139018..3140556	+	1539	WP_001187183.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3125202..3148368	23166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14210.19 Da
• Isoelectric Point: 7.2920

>T97876 WP_000854896.1 NZ_CP027312:3135456-3135833 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T97876 NZ_CP027312:3135456-3135833 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13664.42 Da
• Isoelectric Point: 6.4757

>AT97876 WP_024175300.1 NZ_CP027312:3135035-3135409 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYLADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHSVTLYAKGLTCEADTLGSCGYVYLTVYPTPATATTS

Nucleotide

• Length: 375 bp

>AT97876 NZ_CP027312:3135035-3135409 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAA
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGCGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATTCCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCAACCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0P7PH46

Antitoxin

• AlphaFold DB: A0A234XUW5