Detailed information of TA system TA097874

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2426291..2427092
• Replicon: chromosome
• Accession: NZ_CP027312
• Organism: Escherichia coli strain 2013C-3181

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MJ73_RS12735
• Protein ID: WP_001094440.1
• Coordinates: 2426291..2426668 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MJ73_RS12740
• Protein ID: WP_024166067.1
• Coordinates: 2426715..2427092 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ73_RS12705	2421823..2423142	+	1320	WP_000144687.1	site-specific integrase	-
MJ73_RS12710	2423235..2424083	-	849	WP_001280444.1	DUF4942 domain-containing protein	-
MJ73_RS12720	2424364..2425383	-	1020	WP_000875212.1	IS110 family transposase	-
MJ73_RS12725	2425597..2425794	-	198	WP_000839232.1	DUF957 domain-containing protein	-
MJ73_RS12730	2425806..2426294	-	489	WP_000761703.1	hypothetical protein	-
MJ73_RS12735	2426291..2426668	-	378	WP_001094440.1	TA system toxin CbtA family protein	Toxin
MJ73_RS12740	2426715..2427092	-	378	WP_024166067.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MJ73_RS12745	2427167..2427388	-	222	WP_000692350.1	DUF987 domain-containing protein	-
MJ73_RS12750	2427475..2427951	-	477	WP_001367144.1	RadC family protein	-
MJ73_RS12755	2427967..2428446	-	480	WP_001449312.1	antirestriction protein	-
MJ73_RS12760	2428540..2428785	-	246	WP_000680583.1	hypothetical protein	-
MJ73_RS12765	2428785..2429603	-	819	WP_001234621.1	DUF945 domain-containing protein	-
MJ73_RS12775	2429824..2430234	-	411	WP_000846706.1	hypothetical protein	-
MJ73_RS12780	2430250..2430933	-	684	WP_000775504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	2395724..2455166	59442
	-	flank	IS/Tn	-	-	2424364..2425383	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13961.87 Da
• Isoelectric Point: 7.3225

>T97874 WP_001094440.1 NZ_CP027312:c2426668-2426291 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Nucleotide

• Length: 378 bp

>T97874 NZ_CP027312:c2426668-2426291 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGTCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13676.53 Da
• Isoelectric Point: 5.9505

>AT97874 WP_024166067.1 NZ_CP027312:c2427092-2426715 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT97874 NZ_CP027312:c2427092-2426715 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA