Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120019..120544 Replicon plasmid unnamed
Accession NZ_CP027311
Organism Escherichia coli strain 2014C-4135

Toxin (Protein)


Gene name ccdB Uniprot ID -
Locus tag C6N22_RS26080 Protein ID WP_050866971.1
Coordinates 120239..120544 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C6N22_RS26075 Protein ID WP_000813634.1
Coordinates 120019..120237 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N22_RS26065 116503..117549 + 1047 WP_024232765.1 SIR2 family protein -
C6N22_RS26070 117546..119354 + 1809 WP_044863035.1 ATP-binding protein -
C6N22_RS26075 120019..120237 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6N22_RS26080 120239..120544 + 306 WP_050866971.1 type II toxin-antitoxin system toxin CcdB Toxin
C6N22_RS26085 120545..121354 + 810 WP_000016979.1 site-specific integrase -
C6N22_RS26090 121492..121767 - 276 WP_000239529.1 hypothetical protein -
C6N22_RS26095 121761..122405 - 645 WP_000633911.1 AAA family ATPase -
C6N22_RS26100 122634..123605 + 972 WP_001103694.1 hypothetical protein -
C6N22_RS26105 123610..124002 + 393 WP_000340833.1 plasmid partitioning/stability family protein -
C6N22_RS26115 124007..125272 - 1266 Protein_111 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP 1..133438 133438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11711.53 Da        Isoelectric Point: 7.0118

>T97861 WP_050866971.1 NZ_CP027311:120239-120544 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVRVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T97861 NZ_CP027311:120239-120544 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCGTGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT97861 WP_000813634.1 NZ_CP027311:120019-120237 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT97861 NZ_CP027311:120019-120237 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References