Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4933599..4934217 Replicon chromosome
Accession NZ_CP027310
Organism Escherichia coli strain 2014C-4135

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6N22_RS25400 Protein ID WP_001291435.1
Coordinates 4933999..4934217 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6N22_RS25395 Protein ID WP_000344800.1
Coordinates 4933599..4933973 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N22_RS25385 4928688..4929881 + 1194 WP_044862765.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C6N22_RS25390 4929904..4933053 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C6N22_RS25395 4933599..4933973 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6N22_RS25400 4933999..4934217 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6N22_RS25405 4934389..4934940 + 552 WP_000102568.1 maltose O-acetyltransferase -
C6N22_RS25410 4935056..4935526 + 471 WP_000136192.1 YlaC family protein -
C6N22_RS25415 4935690..4937240 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6N22_RS25420 4937282..4937635 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C6N22_RS25430 4938013..4938324 + 312 WP_000409911.1 MGMT family protein -
C6N22_RS25435 4938355..4938927 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T97856 WP_001291435.1 NZ_CP027310:4933999-4934217 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T97856 NZ_CP027310:4933999-4934217 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT97856 WP_000344800.1 NZ_CP027310:4933599-4933973 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT97856 NZ_CP027310:4933599-4933973 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References