Detailed information of TA system TA097845

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3168358..3169193
• Replicon: chromosome
• Accession: NZ_CP027310
• Organism: Escherichia coli strain 2014C-4135

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E1LX62
• Locus tag: C6N22_RS16095
• Protein ID: WP_000854745.1
• Coordinates: 3168816..3169193 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A479FB04
• Locus tag: C6N22_RS16090
• Protein ID: WP_047651719.1
• Coordinates: 3168358..3168726 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N22_RS16060	3165228..3165683	+	456	WP_047651727.1	hypothetical protein	-
C6N22_RS16065	3165718..3165993	+	276	WP_077875616.1	DUF905 family protein	-
C6N22_RS16070	3166083..3166901	+	819	WP_047651723.1	DUF945 domain-containing protein	-
C6N22_RS16075	3166993..3167478	+	486	WP_047651721.1	antirestriction protein	-
C6N22_RS16080	3167494..3167970	+	477	WP_001186725.1	RadC family protein	-
C6N22_RS16085	3168057..3168278	+	222	WP_000691818.1	DUF987 domain-containing protein	-
C6N22_RS16090	3168358..3168726	+	369	WP_047651719.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6N22_RS16095	3168816..3169193	+	378	WP_000854745.1	TA system toxin CbtA family protein	Toxin
C6N22_RS16100	3169190..3169678	+	489	WP_041124065.1	hypothetical protein	-
C6N22_RS16105	3169690..3169887	+	198	WP_000839242.1	DUF957 domain-containing protein	-
C6N22_RS16110	3170101..3171120	+	1020	WP_000875212.1	IS110 family transposase	-
C6N22_RS16120	3171401..3172242	+	842	Protein_2914	DUF4942 domain-containing protein	-
C6N22_RS16130	3172713..3173651	+	939	WP_000351323.1	glyoxylate/hydroxypyruvate reductase GhrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3170101..3171120	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14071.99 Da
• Isoelectric Point: 7.9308

>T97845 WP_000854745.1 NZ_CP027310:3168816-3169193 [Escherichia coli]
MKTLPDTHVREASRSPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T97845 NZ_CP027310:3168816-3169193 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCAGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCTC
CCGACTACTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13542.26 Da
• Isoelectric Point: 5.9562

>AT97845 WP_047651719.1 NZ_CP027310:3168358-3168726 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPSHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT97845 NZ_CP027310:3168358-3168726 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGCCCCAGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCAGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E1LX62

Antitoxin

• AlphaFold DB: A0A479FB04