Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2289737..2290391 Replicon chromosome
Accession NZ_CP027310
Organism Escherichia coli strain 2014C-4135

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag C6N22_RS11825 Protein ID WP_000244772.1
Coordinates 2289984..2290391 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6N22_RS11820 Protein ID WP_000354046.1
Coordinates 2289737..2290003 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N22_RS11795 2285025..2285768 + 744 WP_000951964.1 SDR family oxidoreductase -
C6N22_RS11800 2285825..2287258 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
C6N22_RS11805 2287303..2287614 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
C6N22_RS11810 2287778..2288437 + 660 WP_000250274.1 hemolysin III family protein -
C6N22_RS11815 2288514..2289494 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6N22_RS11820 2289737..2290003 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6N22_RS11825 2289984..2290391 + 408 WP_000244772.1 protein YgfX Toxin
C6N22_RS11830 2290431..2290952 - 522 WP_001055874.1 flavodoxin FldB -
C6N22_RS11835 2291064..2291960 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6N22_RS11840 2291985..2292695 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6N22_RS11845 2292701..2294434 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T97842 WP_000244772.1 NZ_CP027310:2289984-2290391 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T97842 NZ_CP027310:2289984-2290391 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT97842 WP_000354046.1 NZ_CP027310:2289737-2290003 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT97842 NZ_CP027310:2289737-2290003 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References