Detailed information of TA system TA097839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1628508..1629308
• Replicon: chromosome
• Accession: NZ_CP027310
• Organism: Escherichia coli strain 2014C-4135

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: C6N22_RS08255
• Protein ID: WP_044862839.1
• Coordinates: 1628781..1629308 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: C6N22_RS08250
• Protein ID: WP_001277108.1
• Coordinates: 1628508..1628774 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N22_RS08230	1624166..1624834	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
C6N22_RS08235	1624827..1625885	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
C6N22_RS08240	1626130..1626984	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
C6N22_RS08245	1627255..1628358	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
C6N22_RS08250	1628508..1628774	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
C6N22_RS08255	1628781..1629308	+	528	WP_044862839.1	GNAT family N-acetyltransferase	Toxin
C6N22_RS08260	1629305..1629688	-	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
C6N22_RS08265	1630112..1631221	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
C6N22_RS08270	1631269..1632195	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C6N22_RS08275	1632192..1633469	+	1278	WP_000803771.1	branched chain amino acid ABC transporter permease LivM	-
C6N22_RS08280	1633466..1634233	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19622.59 Da
• Isoelectric Point: 7.3230

>T97839 WP_044862839.1 NZ_CP027310:1628781-1629308 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPESQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T97839 NZ_CP027310:1628781-1629308 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAAAGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT97839 WP_001277108.1 NZ_CP027310:1628508-1628774 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT97839 NZ_CP027310:1628508-1628774 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24