Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 26956..27557 Replicon plasmid unnamed2
Accession NZ_CP027309
Organism Escherichia coli strain 2015C-3108

Toxin (Protein)


Gene name doc Uniprot ID A0A731GHK2
Locus tag C6N27_RS29255 Protein ID WP_001216038.1
Coordinates 26956..27336 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag C6N27_RS29260 Protein ID WP_001190712.1
Coordinates 27336..27557 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N27_RS29220 22836..23864 + 1029 WP_063074802.1 tyrosine-type recombinase/integrase -
C6N27_RS29225 23974..24282 + 309 WP_000349257.1 hypothetical protein -
C6N27_RS29230 24279..24755 + 477 WP_096850650.1 hypothetical protein -
C6N27_RS29235 24752..25246 + 495 WP_000640907.1 dUTP diphosphatase -
C6N27_RS29240 25261..25938 + 678 WP_106895323.1 DUF2829 domain-containing protein -
C6N27_RS29245 25945..26721 + 777 WP_000432096.1 hypothetical protein -
C6N27_RS29250 26754..26951 - 198 WP_000113017.1 hypothetical protein -
C6N27_RS29255 26956..27336 - 381 WP_001216038.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
C6N27_RS29260 27336..27557 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6N27_RS29265 27666..28088 - 423 WP_000098854.1 hypothetical protein -
C6N27_RS29270 28223..28612 - 390 WP_000506729.1 DNA repair protein -
C6N27_RS29275 28624..28770 - 147 WP_001443773.1 hypothetical protein -
C6N27_RS29280 28944..29153 + 210 WP_106895326.1 hypothetical protein -
C6N27_RS29285 29138..29422 - 285 WP_001142397.1 hypothetical protein -
C6N27_RS29290 29406..30572 - 1167 WP_000208025.1 hypothetical protein -
C6N27_RS29295 30569..30778 - 210 WP_000951712.1 hypothetical protein -
C6N27_RS29300 30780..30974 - 195 WP_000224731.1 hypothetical protein -
C6N27_RS29305 30976..31701 - 726 WP_000078034.1 ead/Ea22-like family protein -
C6N27_RS29310 31698..31937 - 240 WP_000476201.1 hypothetical protein -
C6N27_RS29315 31930..32133 - 204 WP_000158003.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93724 93724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13574.27 Da        Isoelectric Point: 5.1514

>T97831 WP_001216038.1 NZ_CP027309:c27336-26956 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEISDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T97831 NZ_CP027309:c27336-26956 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGTGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCAGCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT97831 WP_001190712.1 NZ_CP027309:c27557-27336 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT97831 NZ_CP027309:c27557-27336 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTTAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CCGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A731GHK2

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References