Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3792239..3792736 Replicon chromosome
Accession NZ_CP027307
Organism Escherichia coli strain 2015C-3108

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6N27_RS20525 Protein ID WP_001173343.1
Coordinates 3792239..3792412 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C6N27_RS20530 Protein ID WP_000398619.1
Coordinates 3792440..3792736 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N27_RS20505 3789437..3789598 + 162 Protein_3769 IS256 family transposase -
C6N27_RS20510 3789634..3790065 - 432 Protein_3770 DDE-type integrase/transposase/recombinase -
C6N27_RS20515 3790119..3791332 + 1214 WP_162829202.1 IS3 family transposase -
C6N27_RS20520 3791380..3792120 + 741 Protein_3772 lysine--tRNA ligase -
C6N27_RS20525 3792239..3792412 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6N27_RS20530 3792440..3792736 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6N27_RS20540 3792963..3793235 - 273 WP_000405647.1 N-acetyltransferase -
C6N27_RS20545 3793247..3793477 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
C6N27_RS20550 3793658..3795289 + 1632 WP_001216487.1 sensor histidine kinase -
C6N27_RS20555 3795286..3796005 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / espL2 / nleB1 / nleE / efa1 3771368..3792736 21368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T97823 WP_001173343.1 NZ_CP027307:c3792412-3792239 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T97823 NZ_CP027307:c3792412-3792239 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT97823 WP_000398619.1 NZ_CP027307:c3792736-3792440 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT97823 NZ_CP027307:c3792736-3792440 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References