Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1994506..1994977 Replicon chromosome
Accession NZ_CP027307
Organism Escherichia coli strain 2015C-3108

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6N27_RS10820 Protein ID WP_001303511.1
Coordinates 1994699..1994977 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6N27_RS10815 Protein ID WP_001302048.1
Coordinates 1994506..1994697 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N27_RS10770 1989979..1990251 - 273 WP_012817871.1 hypothetical protein -
C6N27_RS10780 1990419..1990559 - 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
C6N27_RS10785 1990597..1991810 + 1214 WP_162829202.1 IS3 family transposase -
C6N27_RS10790 1991850..1992266 - 417 WP_023981635.1 DUF977 family protein -
C6N27_RS10795 1992307..1993269 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
C6N27_RS10800 1993292..1993717 - 426 WP_000693943.1 Rha family transcriptional regulator -
C6N27_RS10805 1993714..1994016 - 303 WP_001444689.1 transcriptional regulator -
C6N27_RS10810 1994114..1994485 + 372 WP_001169687.1 hypothetical protein -
C6N27_RS10815 1994506..1994697 + 192 WP_001302048.1 hypothetical protein Antitoxin
C6N27_RS10820 1994699..1994977 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6N27_RS10830 1995273..1995662 + 390 WP_001447517.1 hypothetical protein -
C6N27_RS10835 1995703..1995921 + 219 WP_001171930.1 hypothetical protein -
C6N27_RS10845 1996405..1996593 + 189 WP_000449192.1 cell division inhibition protein DicB -
C6N27_RS10850 1996590..1996781 + 192 WP_001090200.1 DUF1482 family protein -
C6N27_RS10855 1996874..1999339 + 2466 WP_106895297.1 exonuclease -
C6N27_RS10860 1999401..1999670 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espN / espX7/nleL 1946686..2007926 61240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T97816 WP_001303511.1 NZ_CP027307:1994699-1994977 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T97816 NZ_CP027307:1994699-1994977 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT97816 WP_001302048.1 NZ_CP027307:1994506-1994697 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT97816 NZ_CP027307:1994506-1994697 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References