Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1524978..1525616 Replicon chromosome
Accession NZ_CP027307
Organism Escherichia coli strain 2015C-3108

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6N27_RS08190 Protein ID WP_000813794.1
Coordinates 1524978..1525154 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6N27_RS08195 Protein ID WP_001270286.1
Coordinates 1525200..1525616 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N27_RS08170 1520603..1521811 - 1209 WP_071532666.1 BenE family transporter YdcO -
C6N27_RS08175 1521864..1522400 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6N27_RS08180 1522473..1524434 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6N27_RS08185 1524526..1524756 - 231 WP_000494244.1 YncJ family protein -
C6N27_RS08190 1524978..1525154 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6N27_RS08195 1525200..1525616 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6N27_RS08200 1525695..1527101 + 1407 WP_000760637.1 PLP-dependent aminotransferase family protein -
C6N27_RS08205 1527346..1528491 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6N27_RS08210 1528509..1529522 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6N27_RS08215 1529523..1530464 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1519415..1520563 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T97814 WP_000813794.1 NZ_CP027307:1524978-1525154 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T97814 NZ_CP027307:1524978-1525154 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT97814 WP_001270286.1 NZ_CP027307:1525200-1525616 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT97814 NZ_CP027307:1525200-1525616 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References