Detailed information of TA system TA097804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 363847..364472
• Replicon: chromosome
• Accession: NZ_CP027307
• Organism: Escherichia coli strain 2015C-3108

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6N27_RS01900
• Protein ID: WP_000911330.1
• Coordinates: 364074..364472 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6N27_RS01895
• Protein ID: WP_000450524.1
• Coordinates: 363847..364074 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N27_RS01870	359638..360108	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6N27_RS01875	360108..360692	-	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
C6N27_RS01880	360838..361716	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6N27_RS01885	361733..362767	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6N27_RS01890	362980..363693	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6N27_RS01895	363847..364074	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6N27_RS01900	364074..364472	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6N27_RS01905	364619..365482	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6N27_RS01910	365497..367512	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6N27_RS01915	367586..368284	+	699	WP_000679812.1	esterase	-
C6N27_RS01920	368394..368594	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T97804 WP_000911330.1 NZ_CP027307:364074-364472 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T97804 NZ_CP027307:364074-364472 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT97804 WP_000450524.1 NZ_CP027307:363847-364074 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT97804 NZ_CP027307:363847-364074 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33