Detailed information of TA system TA097799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3167093..3167657
• Replicon: chromosome
• Accession: NZ_CP027306
• Organism: Streptomyces atratus strain SCSIO_ZH16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C5746_RS14385
• Protein ID: WP_114244521.1
• Coordinates: 3167093..3167440 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1G5J6E9
• Locus tag: C5746_RS14390
• Protein ID: WP_030921349.1
• Coordinates: 3167433..3167657 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5746_RS14375	3162514..3165813	+	3300	WP_114249005.1	S8 family serine peptidase	-
C5746_RS14380	3166006..3167034	+	1029	WP_114244520.1	aspartate-semialdehyde dehydrogenase	-
C5746_RS14385	3167093..3167440	-	348	WP_114244521.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C5746_RS14390	3167433..3167657	-	225	WP_030921349.1	ribbon-helix-helix domain-containing protein	Antitoxin
C5746_RS14395	3167758..3170340	+	2583	WP_114244522.1	aminopeptidase N	-
C5746_RS14400	3170419..3170565	-	147	WP_157987102.1	hypothetical protein	-
C5746_RS14405	3170788..3171927	-	1140	WP_114244524.1	allantoicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12709.74 Da
• Isoelectric Point: 11.7479

>T97799 WP_114244521.1 NZ_CP027306:c3167440-3167093 [Streptomyces atratus]
MRRGDIHLVDLEPARGSEANKVRPAVIVSNNAANQSVELNGRGVITVVPLTSNTARVLTFQVLLRADESRLPKDSKVQCE
QVRAVAPDRVLKRIGTVPRRRMAEIDAALRRHLAL

Nucleotide

• Length: 348 bp

>T97799 NZ_CP027306:c3167440-3167093 [Streptomyces atratus]
ATGCGTAGAGGCGACATCCACCTGGTCGATCTGGAGCCGGCCAGGGGCAGCGAGGCCAACAAGGTCAGGCCGGCCGTGAT
CGTCTCCAACAACGCCGCCAACCAGTCGGTCGAGCTCAACGGCAGAGGCGTGATCACCGTGGTGCCTCTGACGTCGAACA
CCGCACGTGTTCTCACGTTCCAGGTCCTTCTCAGGGCCGACGAGAGCCGTCTGCCGAAGGACTCGAAGGTCCAGTGCGAG
CAGGTCCGGGCGGTCGCCCCGGACCGTGTCCTGAAGCGGATCGGTACCGTCCCGCGCCGGCGCATGGCCGAGATCGACGC
CGCCCTGCGACGGCATCTCGCCCTCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8076.86 Da
• Isoelectric Point: 3.7403

>AT97799 WP_030921349.1 NZ_CP027306:c3167657-3167433 [Streptomyces atratus]
MKISVSLPQEDVAFVDEYAMKTDADSRSAVIHAAIELLRVAGLEAEYTEAFEEWDASEDAALWDRTVGDGIADA

Nucleotide

• Length: 225 bp

>AT97799 NZ_CP027306:c3167657-3167433 [Streptomyces atratus]
ATGAAGATCAGTGTGAGCCTGCCGCAGGAGGATGTCGCCTTCGTCGACGAGTACGCCATGAAGACCGATGCGGATTCCCG
GTCCGCGGTGATCCATGCCGCCATCGAGCTGCTGCGTGTCGCCGGTCTGGAGGCGGAGTACACGGAGGCGTTCGAGGAGT
GGGACGCGAGCGAGGACGCCGCGCTCTGGGACCGTACGGTCGGAGACGGAATCGCCGATGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1G5J6E9