Detailed information of TA system TA097797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 15825..16468
• Replicon: plasmid unnamed_1
• Accession: NZ_CP027301
• Organism: Serratia marcescens strain SGAir0764

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C1N78_RS24745
• Protein ID: WP_110147625.1
• Coordinates: 15825..16241 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: C1N78_RS24750
• Protein ID: WP_110147679.1
• Coordinates: 16238..16468 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1N78_RS24700	11208..12185	+	978	WP_110147620.1	type II toxin-antitoxin system PemK/MazF family toxin	-
C1N78_RS24705	12511..12732	-	222	WP_110147677.1	hypothetical protein	-
C1N78_RS24715	12966..13535	-	570	WP_110147621.1	hypothetical protein	-
C1N78_RS24720	13540..13785	-	246	WP_110147622.1	hypothetical protein	-
C1N78_RS24725	14256..14633	+	378	WP_060560550.1	Ltp family lipoprotein	-
C1N78_RS24730	14742..14783	+	42	WP_110147678.1	DUF1493 family protein	-
C1N78_RS24735	14908..15174	-	267	WP_148666835.1	hypothetical protein	-
C1N78_RS24740	15238..15765	-	528	WP_110147624.1	hypothetical protein	-
C1N78_RS24745	15825..16241	-	417	WP_110147625.1	PIN domain-containing protein	Toxin
C1N78_RS24750	16238..16468	-	231	WP_110147679.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C1N78_RS24755	16722..17855	+	1134	WP_110147626.1	DUF3800 domain-containing protein	-
C1N78_RS24760	17967..18809	+	843	WP_110147627.1	GIY-YIG nuclease family protein	-
C1N78_RS24765	18984..19598	-	615	WP_110147680.1	hypothetical protein	-
C1N78_RS24770	20529..20822	+	294	WP_110147628.1	hypothetical protein	-
C1N78_RS24775	20887..21195	+	309	WP_110147629.1	hypothetical protein	-
C1N78_RS25435	21206..21364	+	159	WP_199561531.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76484	76484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15042.36 Da
• Isoelectric Point: 7.8874

>T97797 WP_110147625.1 NZ_CP027301:c16241-15825 [Serratia marcescens]
VNKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNHRIVISAITYAEMRFGAIGKRASPRHAQLVDAFCARLDAILPWDRT
AVEATSEIKAALAAAGTPIGPNDTAIAGHAIAAGTILVTNNTREFERVAGLALEDWVK

Nucleotide

• Length: 417 bp

>T97797 NZ_CP027301:c16241-15825 [Serratia marcescens]
GTGAACAAGACCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCAGAAGCCGTCATTAAACGTCT
AGAGCAGGCGGTGCTGCGTAACCACCGCATCGTGATCTCGGCCATCACATATGCCGAAATGCGTTTTGGAGCGATCGGCA
AGAGAGCTTCTCCGCGCCACGCGCAGCTAGTTGACGCGTTCTGTGCCCGCCTCGATGCCATCCTGCCCTGGGACCGGACA
GCCGTGGAGGCCACGTCCGAGATCAAGGCCGCCCTGGCAGCGGCCGGGACACCGATCGGCCCGAACGACACAGCGATCGC
TGGCCATGCCATTGCCGCCGGCACCATTCTTGTGACGAATAATACGCGGGAATTTGAGCGGGTAGCGGGGTTGGCACTTG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8678.76 Da
• Isoelectric Point: 4.4432

>AT97797 WP_110147679.1 NZ_CP027301:c16468-16238 [Serratia marcescens]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDNIILRPVRPTWGSFAQLDKADPDFMAERADVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT97797 NZ_CP027301:c16468-16238 [Serratia marcescens]
ATGCGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGACTTTGAGGGGGTGAG
TGAGCTCGAGATCGTCAGGGAAGGGGATAATATAATCCTGCGCCCCGTCCGCCCGACCTGGGGTTCGTTCGCGCAGCTGG
ATAAGGCCGACCCGGACTTTATGGCGGAGCGTGCGGACGTGGTCAGCGACGAAGGACGATTTGACCTGTGA