Detailed information of TA system TA097779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 79441..80084
• Replicon: plasmid pPAR01
• Accession: NZ_CP027261
• Organism: Pectobacterium parmentieri strain IFB5427

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: C5E18_RS24565
• Protein ID: WP_111778950.1
• Coordinates: 79668..80084 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: C5E18_RS24560
• Protein ID: WP_111778951.1
• Coordinates: 79441..79671 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E18_RS24525	74506..74904	-	399	WP_111778957.1	hypothetical protein	-
C5E18_RS24530	74940..75212	-	273	WP_111778956.1	hypothetical protein	-
C5E18_RS24535	75222..75428	-	207	WP_111778977.1	type II toxin-antitoxin system HicB family antitoxin	-
C5E18_RS24540	75502..75831	-	330	WP_111778955.1	hypothetical protein	-
C5E18_RS24545	76383..77321	+	939	WP_111778954.1	hypothetical protein	-
C5E18_RS24550	77392..77571	-	180	WP_127088045.1	hypothetical protein	-
C5E18_RS24555	77703..79205	-	1503	WP_111778952.1	RES family NAD+ phosphorylase	-
C5E18_RS24560	79441..79671	+	231	WP_111778951.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C5E18_RS24565	79668..80084	+	417	WP_111778950.1	type II toxin-antitoxin system VapC family toxin	Toxin
C5E18_RS24570	80126..81004	-	879	WP_111778949.1	restriction endonuclease	-
C5E18_RS24575	81292..81558	+	267	WP_111778948.1	hypothetical protein	-
C5E18_RS24580	81549..82133	+	585	WP_111778947.1	hypothetical protein	-
C5E18_RS24585	82186..82587	+	402	WP_039310721.1	hypothetical protein	-
C5E18_RS24590	82671..83021	+	351	WP_039310723.1	hypothetical protein	-
C5E18_RS24595	83070..83318	+	249	WP_111778946.1	hypothetical protein	-
C5E18_RS24600	83452..84423	+	972	WP_127088046.1	hypothetical protein	-
C5E18_RS24605	84542..84802	+	261	WP_127088047.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101998	101998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15174.54 Da
• Isoelectric Point: 8.2838

>T97779 WP_111778950.1 NZ_CP027261:79668-80084 [Pectobacterium parmentieri]
VNKTYMLDTCICSFIMREQPEAVLKRLAQAVLRSQRIVVSAITYAEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTDIRVTLRQTGRPIGPNDTAIAGHAIAAGAVLVTNNTREFARVPGLELEDWVR

Nucleotide

• Length: 417 bp

>T97779 NZ_CP027261:79668-80084 [Pectobacterium parmentieri]
GTGAACAAAACTTATATGCTCGACACCTGTATCTGCTCATTCATTATGCGTGAGCAGCCAGAAGCCGTACTGAAACGTCT
AGCGCAAGCGGTATTGCGCAGCCAACGCATCGTGGTTTCGGCCATCACTTATGCCGAAATGCGCTTCGGCGCCACCGGTC
CGAAGGCCTCGCCGCGCCATATCGAATTGGTTGACGCGTTCTGTGCCCGTCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTCGACGCCACCACAGACATCCGGGTCACACTGCGGCAGACAGGCAGACCGATCGGCCCGAACGACACGGCGATTGC
CGGGCATGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACAAGAGAGTTTGCGCGGGTGCCCGGTCTGGAGCTGG
AAGACTGGGTAAGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8708.83 Da
• Isoelectric Point: 4.3775

>AT97779 WP_111778951.1 NZ_CP027261:79441-79671 [Pectobacterium parmentieri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAELEKADPDFMAEREVVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT97779 NZ_CP027261:79441-79671 [Pectobacterium parmentieri]
ATGCGAACCGTATCCATTTTCAAAAACGGCAATAACCGTGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGCGTCAG
TGAGCTGGAGATCGTCCGGGAAGGTGACAGCATCATCCTGCGCCCCGTCCGACCGACCTGGGGCTCGTTCGCTGAGCTTG
AGAAGGCCGATCCGGACTTTATGGCAGAGCGGGAGGTCGTTGTCAGCGATGAAGGACGATTTGACCTGTGA