Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3799182..3799708 Replicon chromosome
Accession NZ_CP027260
Organism Pectobacterium parmentieri strain IFB5427

Toxin (Protein)


Gene name ccdB Uniprot ID A0A8B3FHC0
Locus tag C5E18_RS17950 Protein ID WP_033071873.1
Coordinates 3799403..3799708 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID C6C380
Locus tag C5E18_RS17945 Protein ID WP_015855076.1
Coordinates 3799182..3799400 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E18_RS17920 3795390..3796649 + 1260 WP_069704166.1 integrase arm-type DNA-binding domain-containing protein -
C5E18_RS17925 3796755..3796949 - 195 WP_033071869.1 DUF3950 domain-containing protein -
C5E18_RS17930 3797135..3797476 + 342 WP_033071870.1 hypothetical protein -
C5E18_RS24785 3798068..3798238 - 171 WP_154665134.1 hypothetical protein -
C5E18_RS17940 3798380..3799123 + 744 WP_033071872.1 hypothetical protein -
C5E18_RS17945 3799182..3799400 + 219 WP_015855076.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C5E18_RS17950 3799403..3799708 + 306 WP_033071873.1 type II toxin-antitoxin system toxin CcdB Toxin
C5E18_RS17955 3799814..3800351 + 538 Protein_3462 exonuclease SbcC -
C5E18_RS17960 3800449..3801027 - 579 WP_033071875.1 hypothetical protein -
C5E18_RS17965 3801029..3802360 - 1332 WP_033071876.1 hypothetical protein -
C5E18_RS17970 3803396..3803617 + 222 Protein_3465 integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3795223..3803173 7950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11773.64 Da        Isoelectric Point: 4.4978

>T97777 WP_033071873.1 NZ_CP027260:3799403-3799708 [Pectobacterium parmentieri]
MQFIVYQYRRASRYKMFVDVQSDIVETPKRRMVIPLIEAHNLSEKVNNTLFPLIRIDGEDYRLMTTELSSVPVEVMGEVI
ADLGDYADEIKDAINLMFWGI

Download         Length: 306 bp

>T97777 NZ_CP027260:3799403-3799708 [Pectobacterium parmentieri]
ATGCAATTCATTGTTTATCAATACAGACGCGCCAGCCGCTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATAACCTGTCTGAGAAAGTGAATAATACGCTGTTTCCTCTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTACCTGTTGAAGTCATGGGTGAAGTGATT
GCGGATCTTGGCGACTACGCGGATGAGATCAAAGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8178.13 Da        Isoelectric Point: 7.0135

>AT97777 WP_015855076.1 NZ_CP027260:3799182-3799400 [Pectobacterium parmentieri]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Download         Length: 219 bp

>AT97777 NZ_CP027260:3799182-3799400 [Pectobacterium parmentieri]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTACCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
AGTGAATGACGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCGCAGAATGGCTCGTTTGCGGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FHC0

Antitoxin

Source ID Structure
AlphaFold DB A0A250BG46

References