Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 485153..486189 Replicon chromosome
Accession NZ_CP027260
Organism Pectobacterium parmentieri strain IFB5427

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A8B3FNB9
Locus tag C5E18_RS02305 Protein ID WP_033070784.1
Coordinates 485614..486189 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A8B3FHU5
Locus tag C5E18_RS02300 Protein ID WP_033070783.1
Coordinates 485153..485614 (+) Length 154 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E18_RS02265 481760..482296 - 537 WP_033070780.1 ash family protein -
C5E18_RS02270 482293..482493 - 201 WP_014698606.1 AlpA family transcriptional regulator -
C5E18_RS02275 482496..483374 - 879 WP_033070781.1 integrase domain-containing protein -
C5E18_RS02280 483921..484172 - 252 WP_127087855.1 hypothetical protein -
C5E18_RS02285 484387..484461 + 75 Protein_433 type II toxin-antitoxin system RelE/ParE family toxin -
C5E18_RS02295 484630..484848 - 219 Protein_434 hypothetical protein -
C5E18_RS02300 485153..485614 + 462 WP_033070783.1 helix-turn-helix domain-containing protein Antitoxin
C5E18_RS02305 485614..486189 + 576 WP_033070784.1 PIN domain-containing protein Toxin
C5E18_RS02310 486358..487479 - 1122 WP_033070785.1 XRE family transcriptional regulator -
C5E18_RS02315 487466..488290 - 825 WP_127087856.1 hypothetical protein -
C5E18_RS02320 488352..488564 - 213 WP_082218588.1 DUF1133 family protein -
C5E18_RS02325 489106..490368 + 1263 WP_033070786.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 476073..493079 17006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(84-131)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21771.27 Da        Isoelectric Point: 4.8050

>T97772 WP_033070784.1 NZ_CP027260:485614-486189 [Pectobacterium parmentieri]
MNHSPYPVVLDACVIYPNMLRDLLMEVGKSGLYQPKWTATIHDEWQRNLVENVPDLTREKLKRVEDLMNKALPDALVTGF
ENLMEGVSLPDLDDRHVVAAAIRSDSEVIVTFNLKDFPKESLAEFDIEAIHPDEFIADLLDLNQALVLQAVQRQRARLKN
PKKSVDEYFDILLKLGLPMTVKAITAYKLMI

Download         Length: 576 bp

>T97772 NZ_CP027260:485614-486189 [Pectobacterium parmentieri]
ATGAATCATTCGCCTTACCCAGTCGTACTTGATGCCTGTGTCATTTATCCCAACATGCTCCGCGATCTGCTTATGGAGGT
TGGGAAATCGGGTCTGTATCAACCTAAATGGACGGCCACCATTCATGATGAATGGCAAAGAAATTTGGTTGAAAACGTAC
CGGATTTAACGCGTGAAAAATTGAAGCGCGTAGAGGATCTGATGAACAAAGCCCTACCTGATGCTCTTGTAACGGGCTTT
GAAAATCTTATGGAAGGGGTTTCACTCCCAGATCTTGACGATCGCCATGTTGTAGCTGCAGCGATAAGATCCGATTCAGA
GGTGATCGTAACCTTCAATCTTAAAGACTTTCCAAAGGAGAGCCTGGCTGAGTTTGATATTGAAGCAATTCATCCTGATG
AGTTTATCGCTGATTTGCTGGATTTAAATCAAGCCTTAGTTTTACAGGCTGTCCAAAGGCAGCGAGCCCGGCTAAAGAAC
CCCAAAAAATCAGTAGATGAATATTTCGACATACTACTGAAACTAGGCTTGCCGATGACGGTTAAGGCTATAACTGCTTA
TAAATTAATGATTTAG

Antitoxin


Download         Length: 154 a.a.        Molecular weight: 17321.80 Da        Isoelectric Point: 6.1440

>AT97772 WP_033070783.1 NZ_CP027260:485153-485614 [Pectobacterium parmentieri]
MTSSILDNVSLPAQKEIEVALRGQRELATFLTTKFETQKISIHDANDEKHQIELPTSALRLLMNILGELAEGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGKLPHHKTGRHRRVLFADLMRYKAQRETESNEAMQELVDLSQELGFY

Download         Length: 462 bp

>AT97772 NZ_CP027260:485153-485614 [Pectobacterium parmentieri]
ATGACTAGCTCAATTCTGGACAATGTAAGCCTTCCGGCTCAGAAGGAAATTGAGGTTGCGTTACGTGGACAAAGAGAACT
GGCAACATTTCTCACCACAAAGTTCGAAACGCAAAAAATATCGATTCACGATGCAAACGACGAAAAACACCAGATCGAAT
TGCCAACCTCAGCACTGAGATTGCTAATGAACATCTTGGGTGAACTCGCTGAAGGGAACGCGGTTCAAGTTGTCCCTGTG
CACGCTGAATTAACTACACAGGAGGCCGCCAATATTCTGAATGTTTCACGCCCTCACATGGTGAAGCTTTTAGAAGAAGG
AAAGCTTCCACATCATAAAACAGGCCGTCATCGCCGCGTTCTTTTTGCTGATTTGATGCGCTATAAAGCTCAGCGTGAAA
CTGAGAGCAATGAAGCAATGCAAGAACTGGTTGATTTGAGCCAGGAACTTGGTTTTTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNB9

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3FHU5

References