Detailed information of TA system TA097769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 40239..40786
• Replicon: plasmid unnamed1
• Accession: NZ_CP027256
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C6C13_RS25760
• Protein ID: WP_000969978.1
• Coordinates: 40505..40786 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A478PNR7
• Locus tag: C6C13_RS25755
• Protein ID: WP_000195511.1
• Coordinates: 40239..40508 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6C13_RS25700	35635..35823	+	189	WP_001345829.1	hypothetical protein	-
C6C13_RS27070	36021..36107	+	87	Protein_42	endonuclease	-
C6C13_RS25710	36288..36599	-	312	WP_000802277.1	hypothetical protein	-
-	36895..36956	-	62	NuclAT_1	-	-
-	36895..36956	-	62	NuclAT_1	-	-
-	36895..36956	-	62	NuclAT_1	-	-
-	36895..36956	-	62	NuclAT_1	-	-
C6C13_RS25715	36943..37149	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
C6C13_RS25720	37433..37690	+	258	WP_000083834.1	replication regulatory protein RepA	-
C6C13_RS25730	37924..37998	+	75	WP_001442103.1	RepA leader peptide Tap	-
C6C13_RS25735	37991..38473	+	483	WP_001443034.1	hypothetical protein	-
C6C13_RS25740	38466..39323	+	858	WP_000774301.1	incFII family plasmid replication initiator RepA	-
C6C13_RS25755	40239..40508	+	270	WP_000195511.1	hypothetical protein	Antitoxin
C6C13_RS25760	40505..40786	+	282	WP_000969978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6C13_RS25765	40832..41689	+	858	WP_069924904.1	3'-5' exonuclease	-
C6C13_RS25770	41693..42166	+	474	WP_001351578.1	hypothetical protein	-
C6C13_RS25780	43262..44323	-	1062	WP_001092154.1	EAL domain-containing protein	-
C6C13_RS25785	44433..44846	-	414	WP_033561996.1	hypothetical protein	-
C6C13_RS25790	45010..45474	-	465	WP_001351581.1	polymer-forming cytoskeletal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103336	103336

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10832.56 Da
• Isoelectric Point: 8.0078

>T97769 WP_000969978.1 NZ_CP027256:40505-40786 [Escherichia coli]
MMEIFWTILASQDRKCIRGYITEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGIRELVIYPHFVLVYEIDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T97769 NZ_CP027256:40505-40786 [Escherichia coli]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGTGCATTCGTGGATACATTACGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGACAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTATCCGGGAACTGGTTATTTACCCGCATTTTGTTCTGGTTTATGAGATCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10240.57 Da
• Isoelectric Point: 8.8735

>AT97769 WP_000195511.1 NZ_CP027256:40239-40508 [Escherichia coli]
MTQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT97769 NZ_CP027256:40239-40508 [Escherichia coli]
ATGACACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A478PNR7