Detailed information of TA system TA097764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4380098..4380624
• Replicon: chromosome
• Accession: NZ_CP027255
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: C6C13_RS22465
• Protein ID: WP_000323025.1
• Coordinates: 4380337..4380624 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: C6C13_RS22460
• Protein ID: WP_000534858.1
• Coordinates: 4380098..4380337 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6C13_RS22425	4375460..4375816	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
C6C13_RS27015	4376427..4376714	+	288	Protein_4099	hypothetical protein	-
C6C13_RS22440	4377483..4378796	+	1314	WP_033803317.1	ISNCY family transposase	-
C6C13_RS22450	4379233..4379565	-	333	WP_001301033.1	protein FlxA	-
C6C13_RS22455	4379768..4380073	-	306	WP_001326990.1	hypothetical protein	-
C6C13_RS22460	4380098..4380337	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
C6C13_RS22465	4380337..4380624	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
C6C13_RS22470	4380696..4380851	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
C6C13_RS22475	4381068..4381319	+	252	WP_000980994.1	hypothetical protein	-
C6C13_RS22480	4381386..4381664	+	279	WP_012304870.1	hypothetical protein	-
C6C13_RS22485	4381666..4382715	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
C6C13_RS22490	4382729..4383112	+	384	Protein_4109	antitermination protein	-
C6C13_RS22495	4383183..4384360	+	1178	WP_085968792.1	IS3 family transposase	-
C6C13_RS22500	4384371..4384742	+	372	Protein_4111	antitermination protein	-
C6C13_RS26785	4385020..4385109	-	90	WP_120795389.1	hypothetical protein	-
C6C13_RS22505	4385164..4385376	-	213	WP_000087756.1	cold shock-like protein CspF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4366716..4420013	53297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T97764 WP_000323025.1 NZ_CP027255:4380337-4380624 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T97764 NZ_CP027255:4380337-4380624 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT97764 WP_000534858.1 NZ_CP027255:4380098-4380337 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT97764 NZ_CP027255:4380098-4380337 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8