Detailed information of TA system TA097752

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2378725..2379557
• Replicon: chromosome
• Accession: NZ_CP027255
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C6C13_RS12120
• Protein ID: WP_032209244.1
• Coordinates: 2379183..2379557 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6C13_RS12115
• Protein ID: WP_041328523.1
• Coordinates: 2378725..2379093 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6C13_RS12075	2375275..2375730	+	456	WP_001518314.1	hypothetical protein	-
C6C13_RS12080	2375809..2376042	+	234	WP_001119729.1	DUF905 family protein	-
C6C13_RS12090	2376142..2376963	+	822	WP_106022951.1	DUF945 domain-containing protein	-
C6C13_RS12095	2376963..2377208	+	246	WP_041328519.1	hypothetical protein	-
C6C13_RS12100	2377302..2377781	+	480	WP_041328521.1	antirestriction protein	-
C6C13_RS12105	2377796..2378272	+	477	WP_001186774.1	RadC family protein	-
C6C13_RS12110	2378341..2378562	+	222	WP_000692320.1	DUF987 domain-containing protein	-
C6C13_RS12115	2378725..2379093	+	369	WP_041328523.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6C13_RS12120	2379183..2379557	+	375	WP_032209244.1	type IV toxin-antitoxin system toxin CbtA	Toxin
C6C13_RS12125	2379554..2380045	+	492	WP_123000710.1	hypothetical protein	-
C6C13_RS12130	2380057..2380254	+	198	WP_041328527.1	DUF957 domain-containing protein	-
C6C13_RS26965	2380339..2380491	+	153	WP_162862023.1	hypothetical protein	-
C6C13_RS12140	2380955..2382220	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-
C6C13_RS12145	2382421..2383890	-	1470	WP_001022619.1	serine/threonine protein kinase	-
C6C13_RS12150	2383887..2384300	-	414	WP_001358348.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13886.89 Da
• Isoelectric Point: 7.8522

>T97752 WP_032209244.1 NZ_CP027255:2379183-2379557 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T97752 NZ_CP027255:2379183-2379557 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCGCGTCCGTCTCCTGTTGAAATCTGGCAGACACTGCTGTC
CCGCCTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTTGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACTGACCAGCCCGGGTTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14024.89 Da
• Isoelectric Point: 6.4637

>AT97752 WP_041328523.1 NZ_CP027255:2378725-2379093 [Escherichia coli]
VSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYLADRACIRGRFRDADVYHLDQAFPLLMKQLELMLTSG
ELNPRHQYTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT97752 NZ_CP027255:2378725-2379093 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGTGCCTGTATCAGAGGGCGGT
TCAGAGATGCGGATGTGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGTATACCGTCACGCTGTATGCTAAAGGACTGACCTGCGAAGCAGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA