Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 96723..97311 | Replicon | plasmid pOXA58_100004 |
| Accession | NZ_CP027249 | ||
| Organism | Acinetobacter pittii strain WCHAP100004 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | C6N19_RS01060 | Protein ID | WP_000438825.1 |
| Coordinates | 97024..97311 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | C6N19_RS01055 | Protein ID | WP_004728120.1 |
| Coordinates | 96723..97037 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C6N19_RS01020 | 92179..93654 | - | 1476 | WP_000052512.1 | ABC-F type ribosomal protection protein Msr(E) | - |
| C6N19_RS01025 | 94147..94419 | - | 273 | WP_000369781.1 | helix-turn-helix domain-containing protein | - |
| C6N19_RS01030 | 94412..94732 | - | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C6N19_RS01035 | 94872..95291 | + | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| C6N19_RS01040 | 95371..95652 | - | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| C6N19_RS01045 | 95653..95955 | - | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| C6N19_RS01050 | 96104..96634 | + | 531 | WP_000172759.1 | hypothetical protein | - |
| C6N19_RS01055 | 96723..97037 | - | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| C6N19_RS01060 | 97024..97311 | - | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| C6N19_RS01065 | 97541..98146 | - | 606 | WP_000447788.1 | LysE family translocator | - |
| C6N19_RS01070 | 98196..99023 | - | 828 | WP_000073658.1 | AraC family transcriptional regulator | - |
| C6N19_RS01075 | 99151..99903 | - | 753 | Protein_102 | IS1-like element ISAba3 family transposase | - |
| C6N19_RS01080 | 99994..100836 | - | 843 | WP_002002480.1 | carbapenem-hydrolyzing class D beta-lactamase OXA-58 | - |
| C6N19_RS01085 | 101041..101745 | + | 705 | WP_001067790.1 | IS6-like element IS1008 family transposase | - |
| C6N19_RS01095 | 101799..102113 | - | 315 | Protein_105 | chromate resistance protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(3)-IId / floR / sul2 / aph(3'')-Ib / aph(3')-VIb / blaPER-1 / aph(6)-Id / mph(E) / msr(E) / blaOXA-58 | - | 1..105591 | 105591 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T97736 WP_000438825.1 NZ_CP027249:c97311-97024 [Acinetobacter pittii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T97736 NZ_CP027249:c97311-97024 [Acinetobacter pittii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTGACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT97736 WP_004728120.1 NZ_CP027249:c97037-96723 [Acinetobacter pittii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT97736 NZ_CP027249:c97037-96723 [Acinetobacter pittii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACCGATTGGAGCAGTGCTGTACGCC
AAAATCAATTTTACCGCCCAGTGAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |