Detailed information of TA system TA097735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 95371..95955
• Replicon: plasmid pOXA58_100004
• Accession: NZ_CP027249
• Organism: Acinetobacter pittii strain WCHAP100004

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: C6N19_RS01045
• Protein ID: WP_000286964.1
• Coordinates: 95653..95955 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6N19_RS01040
• Protein ID: WP_000985609.1
• Coordinates: 95371..95652 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N19_RS01015	91227..92123	-	897	WP_106405803.1	Mph(E) family macrolide 2'-phosphotransferase	-
C6N19_RS01020	92179..93654	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C6N19_RS01025	94147..94419	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C6N19_RS01030	94412..94732	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N19_RS01035	94872..95291	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C6N19_RS01040	95371..95652	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
C6N19_RS01045	95653..95955	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N19_RS01050	96104..96634	+	531	WP_000172759.1	hypothetical protein	-
C6N19_RS01055	96723..97037	-	315	WP_004728120.1	BrnA antitoxin family protein	-
C6N19_RS01060	97024..97311	-	288	WP_000438825.1	BrnT family toxin	-
C6N19_RS01065	97541..98146	-	606	WP_000447788.1	LysE family translocator	-
C6N19_RS01070	98196..99023	-	828	WP_000073658.1	AraC family transcriptional regulator	-
C6N19_RS01075	99151..99903	-	753	Protein_102	IS1-like element ISAba3 family transposase	-
C6N19_RS01080	99994..100836	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / floR / sul2 / aph(3'')-Ib / aph(3')-VIb / blaPER-1 / aph(6)-Id / mph(E) / msr(E) / blaOXA-58	-	1..105591	105591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T97735 WP_000286964.1 NZ_CP027249:c95955-95653 [Acinetobacter pittii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T97735 NZ_CP027249:c95955-95653 [Acinetobacter pittii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT97735 WP_000985609.1 NZ_CP027249:c95652-95371 [Acinetobacter pittii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT97735 NZ_CP027249:c95652-95371 [Acinetobacter pittii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94