Detailed information of TA system TA097730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 21700..22228
• Replicon: plasmid p1_100004
• Accession: NZ_CP027247
• Organism: Acinetobacter pittii strain WCHAP100004

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0V430
• Locus tag: C6N19_RS00135
• Protein ID: WP_000221360.1
• Coordinates: 21935..22228 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0V429
• Locus tag: C6N19_RS00130
• Protein ID: WP_000246757.1
• Coordinates: 21700..21945 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N19_RS00095	16844..17128	+	285	WP_000155817.1	antibiotic biosynthesis monooxygenase	-
C6N19_RS00100	17263..17484	+	222	Protein_19	IS3 family transposase	-
C6N19_RS00105	17894..18412	+	519	WP_000447196.1	sel1 repeat family protein	-
C6N19_RS00110	18544..19056	+	513	WP_001089570.1	SMI1/KNR4 family protein	-
C6N19_RS00115	19264..19698	+	435	WP_106405795.1	hypothetical protein	-
C6N19_RS00125	20886..21443	+	558	Protein_24	hypothetical protein	-
C6N19_RS00130	21700..21945	+	246	WP_000246757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C6N19_RS00135	21935..22228	+	294	WP_000221360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N19_RS00140	22415..22531	+	117	Protein_27	transposase	-
C6N19_RS00150	23546..23854	+	309	WP_000269910.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N19_RS00155	23895..24197	+	303	WP_001140619.1	XRE family transcriptional regulator	-
C6N19_RS00160	24190..24399	+	210	WP_000069470.1	hypothetical protein	-
C6N19_RS00165	24509..24736	-	228	WP_000555567.1	hypothetical protein	-
C6N19_RS00170	24868..25671	-	804	WP_106405796.1	WYL domain-containing protein	-
C6N19_RS00175	25897..26225	+	329	Protein_33	DUF4236 domain-containing protein	-
C6N19_RS00180	26467..26979	-	513	WP_000464612.1	Fic family protein	-
C6N19_RS00185	26982..27167	-	186	WP_000755724.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66765	66765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11317.49 Da
• Isoelectric Point: 10.4753

>T97730 WP_000221360.1 NZ_CP027247:21935-22228 [Acinetobacter pittii]
MTYKLLRNKDFTAEWEKLPSAIRDQFKKKLAKVIEQPHIPKNMLRGDLTGCYKIKLLKTGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T97730 NZ_CP027247:21935-22228 [Acinetobacter pittii]
ATGACTTATAAGCTTTTACGTAATAAGGACTTCACAGCTGAATGGGAAAAGCTTCCTAGTGCTATAAGAGATCAATTTAA
GAAGAAACTCGCCAAGGTCATAGAGCAGCCACATATTCCCAAGAATATGCTTAGAGGTGATCTCACGGGATGTTATAAAA
TTAAATTACTAAAGACTGGAGTTCGACTGGTCTATCAAGTCAAAGATGATCAGGTAGTTATCTTGCTCATCACCGTTGGG
AAACGAGCTGACAGCATAGTTTATGACGAAGCAAAAAAACGCATTAAAGACTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9264.63 Da
• Isoelectric Point: 4.2821

>AT97730 WP_000246757.1 NZ_CP027247:21700-21945 [Acinetobacter pittii]
MATVNFRVDEALKEKSYSVLREQGIAPTEFFTNVLEYIAATGKLPVQKALLSEEDTELLAIVRKRMNDPKEMFEEITLDD
L

Nucleotide

• Length: 246 bp

>AT97730 NZ_CP027247:21700-21945 [Acinetobacter pittii]
ATGGCTACTGTTAACTTCAGGGTTGATGAAGCCCTTAAAGAAAAATCTTATTCAGTTCTTAGAGAACAAGGTATTGCACC
TACTGAGTTTTTTACAAATGTCCTTGAATACATTGCAGCGACTGGGAAACTCCCTGTACAAAAAGCTTTACTCTCAGAAG
AGGATACCGAGCTATTAGCGATTGTTCGTAAACGTATGAATGATCCTAAAGAAATGTTCGAGGAAATTACTTTAGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: A0A829RR87

Antitoxin

• AlphaFold DB: A0A009MZ79