Detailed information of TA system TA097728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 50198..50783
• Replicon: plasmid pOXA58_005078
• Accession: NZ_CP027245
• Organism: Acinetobacter baumannii strain WCHAB005078

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N9M3U8
• Locus tag: C6N18_RS00835
• Protein ID: WP_005155676.1
• Coordinates: 50496..50783 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9LW73
• Locus tag: C6N18_RS00830
• Protein ID: WP_005155677.1
• Coordinates: 50198..50509 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N18_RS00805	45413..46289	+	877	Protein_49	IS3 family transposase	-
C6N18_RS00810	46537..47751	+	1215	WP_000214119.1	chloramphenicol/florfenicol efflux MFS transporter FloR	-
C6N18_RS00815	47779..48045	+	267	WP_077170324.1	LysR family transcriptional regulator	-
C6N18_RS00820	48081..48785	-	705	WP_005028420.1	IS6 family transposase	-
C6N18_RS00825	49028..50119	+	1092	Protein_53	IS4 family transposase	-
C6N18_RS00830	50198..50509	-	312	WP_005155677.1	BrnA antitoxin family protein	Antitoxin
C6N18_RS00835	50496..50783	-	288	WP_005155676.1	BrnT family toxin	Toxin
C6N18_RS00840	51086..52119	+	1034	Protein_56	ISNCY family transposase	-
C6N18_RS00845	52122..52316	-	195	Protein_57	IS4 family transposase	-
C6N18_RS00850	52320..53045	-	726	Protein_58	IS1 family transposase	-
C6N18_RS00855	53106..53342	-	237	Protein_59	IS4 family transposase	-
C6N18_RS00860	53469..54368	-	900	WP_005166156.1	ParB/RepB/Spo0J family partition protein	-
C6N18_RS00865	54387..55163	-	777	WP_005166158.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..70509	70509
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	41726..53345	11619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11275.66 Da
• Isoelectric Point: 5.6113

>T97728 WP_005155676.1 NZ_CP027245:c50783-50496 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T97728 NZ_CP027245:c50783-50496 [Acinetobacter baumannii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTGTTTTTGAAGATCCTCTACGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAGCGTTGGCAAACCATTGGTA
AGGTTAAAGGCGTACTGATGTTATTAGTCGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCGGAGCGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11984.58 Da
• Isoelectric Point: 6.7277

>AT97728 WP_005155677.1 NZ_CP027245:c50509-50198 [Acinetobacter baumannii]
MSMVRYTREELNENFSNKQDAEVEHLLAKGTVPDDQLDLSDIPEITDWSNAIRHGQFFRPVKQQTSVRLDADVLAWLKAQ
GKGYQTRMNKILREAMLKDLKNH

Nucleotide

• Length: 312 bp

>AT97728 NZ_CP027245:c50509-50198 [Acinetobacter baumannii]
ATGAGCATGGTTAGATATACACGCGAAGAGCTGAATGAAAATTTCAGCAACAAGCAAGATGCTGAAGTTGAACACTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTTTCGTCCAGTGAAGCAACAAACGTCAGTTCGTCTTGATGCGGATGTATTAGCATGGTTAAAAGCACAA
GGAAAAGGTTATCAAACACGTATGAATAAAATTCTGCGTGAAGCAATGCTAAAAGATTTAAAAAATCATTAA

Structures

Toxin

• AlphaFold DB: N9M3U8

Antitoxin

• AlphaFold DB: N9LW73