Detailed information of TA system TA097726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 39425..40009
• Replicon: plasmid pOXA58_005078
• Accession: NZ_CP027245
• Organism: Acinetobacter baumannii strain WCHAB005078

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: C6N18_RS00750
• Protein ID: WP_000286964.1
• Coordinates: 39425..39727 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6N18_RS00755
• Protein ID: WP_000985609.1
• Coordinates: 39728..40009 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N18_RS00715	35237..35941	-	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
C6N18_RS00725	36006..36959	-	954	Protein_35	HAMP domain-containing protein	-
C6N18_RS00730	36949..37677	-	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
C6N18_RS00735	38069..38356	+	288	WP_000438826.1	BrnT family toxin	-
C6N18_RS00740	38343..38657	+	315	WP_001983304.1	BrnA antitoxin family protein	-
C6N18_RS00745	38746..39276	-	531	WP_000172759.1	hypothetical protein	-
C6N18_RS00750	39425..39727	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N18_RS00755	39728..40009	+	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
C6N18_RS00760	40089..40508	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C6N18_RS00765	40648..40968	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N18_RS00770	40961..41233	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C6N18_RS00775	41726..43201	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C6N18_RS00780	43257..44141	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C6N18_RS00785	44332..44580	-	249	WP_087548421.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..70509	70509
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	41726..53345	11619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T97726 WP_000286964.1 NZ_CP027245:39425-39727 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T97726 NZ_CP027245:39425-39727 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT97726 WP_000985609.1 NZ_CP027245:39728-40009 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT97726 NZ_CP027245:39728-40009 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TV94