Detailed information of TA system TA097725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 38069..38657
• Replicon: plasmid pOXA58_005078
• Accession: NZ_CP027245
• Organism: Acinetobacter baumannii strain WCHAB005078

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: C6N18_RS00735
• Protein ID: WP_000438826.1
• Coordinates: 38069..38356 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: C6N18_RS00740
• Protein ID: WP_001983304.1
• Coordinates: 38343..38657 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N18_RS00700	33358..34110	-	753	Protein_32	IS1-like element ISAba3 family transposase	-
C6N18_RS00705	34201..35043	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
C6N18_RS00715	35237..35941	-	705	WP_001067790.1	IS6-like element IS1008 family transposase	-
C6N18_RS00725	36006..36959	-	954	Protein_35	HAMP domain-containing protein	-
C6N18_RS00730	36949..37677	-	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
C6N18_RS00735	38069..38356	+	288	WP_000438826.1	BrnT family toxin	Toxin
C6N18_RS00740	38343..38657	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
C6N18_RS00745	38746..39276	-	531	WP_000172759.1	hypothetical protein	-
C6N18_RS00750	39425..39727	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N18_RS00755	39728..40009	+	282	WP_000985609.1	putative addiction module antidote protein	-
C6N18_RS00760	40089..40508	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C6N18_RS00765	40648..40968	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N18_RS00770	40961..41233	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
C6N18_RS00775	41726..43201	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / msr(E) / mph(E) / floR	-	1..70509	70509
	-	inside	IScluster/Tn	msr(E) / mph(E) / floR	-	41726..53345	11619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T97725 WP_000438826.1 NZ_CP027245:38069-38356 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T97725 NZ_CP027245:38069-38356 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT97725 WP_001983304.1 NZ_CP027245:38343-38657 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT97725 NZ_CP027245:38343-38657 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGAATGAATGAAATTTTACGGGATGCTATGCTGAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3